Detection of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins by sensitivity- enhanced NMR spectroscopy

Aizhou Liu, Weidong Hu, Ananya Majumdar, Michael K. Rosen, Dinshaw J. Patel

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14 Scopus citations

Abstract

We describe the direct observation of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins with sensitivity-enhanced NMR spectroscopy. Specifically, the remote correlation between the hydrogen acceptor side chain carboxylate carbon 13CO2(δ) of glutamate 54 and the hydrogen donor backbone amide 15N of methionine 49 in a 12 kDa protein, human FKBP12, is detected via the trans- hydrogen bond (3h)J(NCO2δ) coupling by employing a novel sensitivity- enhanced HNCO-type experiment, CPD-HNCO. The (3h)J(NCO2δ) coupling constant appears to be even smaller than the average value of backbone (3h)J(NC') couplings, consistent with more extensive local dynamics in protein side chains.

Original languageEnglish (US)
Pages (from-to)79-82
Number of pages4
JournalJournal of Biomolecular NMR
Volume17
Issue number1
DOIs
StatePublished - Jan 1 2000

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Keywords

  • FKBP12
  • NMR detection
  • Sensitivity enhancement
  • Side chain-main chain hydrogen bonds

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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