Detergent-free caveolae proteome suggests an interaction with ER and mitochondria

Kerrie Ann McMahon, Meifang Zhu, Sung Won Kwon, Pingsheng Liu, Yingming Zhao, Richard G W Anderson

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Recent proteomic studies of detergent resistant membrane fractions have begun to characterize the protein composition of caveolae and lipid rafts. The methods used in most of these studies, however, are not able to distinguish between plasma membrane and internal membrane lipid domains. Here we used a non-detergent method for obtaining fractions enriched in caveolae derived from the plasma membrane of multiple cell types. Unexpectedly, the proteins in the caveolae proteome suggest these lipid domains may interact with elements of ER and mitochondria. A comparison of the partial proteome we obtained with other published reports identifies 26 proteins that are candidate marker proteins for identifying caveolae in multiple cell types.

Original languageEnglish (US)
Pages (from-to)143-152
Number of pages10
JournalProteomics
Volume6
Issue number1
DOIs
StatePublished - Jan 2006

Keywords

  • Cell signaling
  • Endoplasmic reticulum
  • Lipid raft
  • Mass spectrometry
  • Membrane proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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