Detergent-free caveolae proteome suggests an interaction with ER and mitochondria

Kerrie Ann McMahon, Meifang Zhu, Sung Won Kwon, Pingsheng Liu, Yingming Zhao, Richard G W Anderson

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Recent proteomic studies of detergent resistant membrane fractions have begun to characterize the protein composition of caveolae and lipid rafts. The methods used in most of these studies, however, are not able to distinguish between plasma membrane and internal membrane lipid domains. Here we used a non-detergent method for obtaining fractions enriched in caveolae derived from the plasma membrane of multiple cell types. Unexpectedly, the proteins in the caveolae proteome suggest these lipid domains may interact with elements of ER and mitochondria. A comparison of the partial proteome we obtained with other published reports identifies 26 proteins that are candidate marker proteins for identifying caveolae in multiple cell types.

Original languageEnglish (US)
Pages (from-to)143-152
Number of pages10
JournalProteomics
Volume6
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Caveolae
Mitochondria
Proteome
Detergents
Cell membranes
Proteins
Cell Membrane
Lipids
Membrane Lipids
Proteomics
Membranes
Chemical analysis

Keywords

  • Cell signaling
  • Endoplasmic reticulum
  • Lipid raft
  • Mass spectrometry
  • Membrane proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

McMahon, K. A., Zhu, M., Kwon, S. W., Liu, P., Zhao, Y., & Anderson, R. G. W. (2006). Detergent-free caveolae proteome suggests an interaction with ER and mitochondria. Proteomics, 6(1), 143-152. https://doi.org/10.1002/pmic.200500208

Detergent-free caveolae proteome suggests an interaction with ER and mitochondria. / McMahon, Kerrie Ann; Zhu, Meifang; Kwon, Sung Won; Liu, Pingsheng; Zhao, Yingming; Anderson, Richard G W.

In: Proteomics, Vol. 6, No. 1, 01.2006, p. 143-152.

Research output: Contribution to journalArticle

McMahon, KA, Zhu, M, Kwon, SW, Liu, P, Zhao, Y & Anderson, RGW 2006, 'Detergent-free caveolae proteome suggests an interaction with ER and mitochondria', Proteomics, vol. 6, no. 1, pp. 143-152. https://doi.org/10.1002/pmic.200500208
McMahon, Kerrie Ann ; Zhu, Meifang ; Kwon, Sung Won ; Liu, Pingsheng ; Zhao, Yingming ; Anderson, Richard G W. / Detergent-free caveolae proteome suggests an interaction with ER and mitochondria. In: Proteomics. 2006 ; Vol. 6, No. 1. pp. 143-152.
@article{f9f41e6f94f04774b657eb7edbaefa91,
title = "Detergent-free caveolae proteome suggests an interaction with ER and mitochondria",
abstract = "Recent proteomic studies of detergent resistant membrane fractions have begun to characterize the protein composition of caveolae and lipid rafts. The methods used in most of these studies, however, are not able to distinguish between plasma membrane and internal membrane lipid domains. Here we used a non-detergent method for obtaining fractions enriched in caveolae derived from the plasma membrane of multiple cell types. Unexpectedly, the proteins in the caveolae proteome suggest these lipid domains may interact with elements of ER and mitochondria. A comparison of the partial proteome we obtained with other published reports identifies 26 proteins that are candidate marker proteins for identifying caveolae in multiple cell types.",
keywords = "Cell signaling, Endoplasmic reticulum, Lipid raft, Mass spectrometry, Membrane proteins",
author = "McMahon, {Kerrie Ann} and Meifang Zhu and Kwon, {Sung Won} and Pingsheng Liu and Yingming Zhao and Anderson, {Richard G W}",
year = "2006",
month = "1",
doi = "10.1002/pmic.200500208",
language = "English (US)",
volume = "6",
pages = "143--152",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "1",

}

TY - JOUR

T1 - Detergent-free caveolae proteome suggests an interaction with ER and mitochondria

AU - McMahon, Kerrie Ann

AU - Zhu, Meifang

AU - Kwon, Sung Won

AU - Liu, Pingsheng

AU - Zhao, Yingming

AU - Anderson, Richard G W

PY - 2006/1

Y1 - 2006/1

N2 - Recent proteomic studies of detergent resistant membrane fractions have begun to characterize the protein composition of caveolae and lipid rafts. The methods used in most of these studies, however, are not able to distinguish between plasma membrane and internal membrane lipid domains. Here we used a non-detergent method for obtaining fractions enriched in caveolae derived from the plasma membrane of multiple cell types. Unexpectedly, the proteins in the caveolae proteome suggest these lipid domains may interact with elements of ER and mitochondria. A comparison of the partial proteome we obtained with other published reports identifies 26 proteins that are candidate marker proteins for identifying caveolae in multiple cell types.

AB - Recent proteomic studies of detergent resistant membrane fractions have begun to characterize the protein composition of caveolae and lipid rafts. The methods used in most of these studies, however, are not able to distinguish between plasma membrane and internal membrane lipid domains. Here we used a non-detergent method for obtaining fractions enriched in caveolae derived from the plasma membrane of multiple cell types. Unexpectedly, the proteins in the caveolae proteome suggest these lipid domains may interact with elements of ER and mitochondria. A comparison of the partial proteome we obtained with other published reports identifies 26 proteins that are candidate marker proteins for identifying caveolae in multiple cell types.

KW - Cell signaling

KW - Endoplasmic reticulum

KW - Lipid raft

KW - Mass spectrometry

KW - Membrane proteins

UR - http://www.scopus.com/inward/record.url?scp=31344468377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31344468377&partnerID=8YFLogxK

U2 - 10.1002/pmic.200500208

DO - 10.1002/pmic.200500208

M3 - Article

C2 - 16294311

AN - SCOPUS:31344468377

VL - 6

SP - 143

EP - 152

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 1

ER -