Determinants of clathrin-coated vesicle acidification.

X. S. Xie, D. K. Stone, E. Racker

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The proton-translocating ATPase of clathrin-coated vesicles of bovine brain is characterized by ATP specificity. Chloride or bromide serve as co-ions balancing electrogenic proton pumping. ATP-driven proton translocation can be observed in the absence of chloride, provided the membrane potential is collapsed by K+ moving out in the presence of valinomycin. Chloride transport can be observed independent of proton movements in the absence of ATP, provided an inside positive membrane potential is generated by K+ moving from the outside to the inside in the presence of valinomycin.

Original languageEnglish (US)
Pages (from-to)14834-14838
Number of pages5
JournalJournal of Biological Chemistry
Volume258
Issue number24
StatePublished - Dec 25 1983

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Clathrin-Coated Vesicles
Clathrin
Acidification
Valinomycin
Protons
Chlorides
Adenosine Triphosphate
Membrane Potentials
Membranes
Proton-Translocating ATPases
Bromides
Brain
Ions

ASJC Scopus subject areas

  • Biochemistry

Cite this

Determinants of clathrin-coated vesicle acidification. / Xie, X. S.; Stone, D. K.; Racker, E.

In: Journal of Biological Chemistry, Vol. 258, No. 24, 25.12.1983, p. 14834-14838.

Research output: Contribution to journalArticle

Xie, XS, Stone, DK & Racker, E 1983, 'Determinants of clathrin-coated vesicle acidification.', Journal of Biological Chemistry, vol. 258, no. 24, pp. 14834-14838.
Xie, X. S. ; Stone, D. K. ; Racker, E. / Determinants of clathrin-coated vesicle acidification. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 24. pp. 14834-14838.
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