Abstract
The proton-translocating ATPase of clathrin-coated vesicles of bovine brain is characterized by ATP specificity. Chloride or bromide serve as co-ions balancing electrogenic proton pumping. ATP-driven proton translocation can be observed in the absence of chloride, provided the membrane potential is collapsed by K+ moving out in the presence of valinomycin. Chloride transport can be observed independent of proton movements in the absence of ATP, provided an inside positive membrane potential is generated by K+ moving from the outside to the inside in the presence of valinomycin.
Original language | English (US) |
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Pages (from-to) | 14834-14838 |
Number of pages | 5 |
Journal | Journal of Biological Chemistry |
Volume | 258 |
Issue number | 24 |
State | Published - Dec 25 1983 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology