Abstract
A rapid and novel ultra-performance liquid chromatography/quadrupole-time of flight mass spectrometry (UPLC/Q-TOFMS) method was developed for the detection of seven biogenic amines in Korean Bokbunja wines. The biogenic amines were pre-column derivatised with dansyl chloride and subsequently extracted by a solid phase extraction cartridge. For the analysis, the dansylated amines were separated on an Acquity™ UPLC column and detected with a micrOTOF-Q II mass spectrometer. The method was validated in terms of linearity, sensitivity, precision and recovery. The coefficient of determination (R 2) was higher than 0.994 for all calibration curves. The limit of detection was between 3 and 15 ng/mL. The intra- and inter-day precisions were below 5.0% and 10.7%, respectively. The proposed method was successfully applied for the biogenic amine analysis of 18 commercially available Bokbunja wines. None of the biogenic amine levels found in Bokbunja wines surpassed the toxic levels reported in the literature.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1185-1190 |
| Number of pages | 6 |
| Journal | Food Chemistry |
| Volume | 132 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 1 2012 |
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Keywords
- Biogenic amine
- Bokbunja wine
- Dansyl chloride
- Q-TOFMS
- UPLC
ASJC Scopus subject areas
- Food Science
- Analytical Chemistry
Cite this
Determination of biogenic amines in Bokbunja (Rubus coreanus Miq.) wines using a novel ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry. / Jia, Shaodong; Kang, Yun Pyo; Park, Jeong Hill; Lee, Jeongmi; Kwon, Sung Won.
In: Food Chemistry, Vol. 132, No. 3, 01.06.2012, p. 1185-1190.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Determination of biogenic amines in Bokbunja (Rubus coreanus Miq.) wines using a novel ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry
AU - Jia, Shaodong
AU - Kang, Yun Pyo
AU - Park, Jeong Hill
AU - Lee, Jeongmi
AU - Kwon, Sung Won
PY - 2012/6/1
Y1 - 2012/6/1
N2 - A rapid and novel ultra-performance liquid chromatography/quadrupole-time of flight mass spectrometry (UPLC/Q-TOFMS) method was developed for the detection of seven biogenic amines in Korean Bokbunja wines. The biogenic amines were pre-column derivatised with dansyl chloride and subsequently extracted by a solid phase extraction cartridge. For the analysis, the dansylated amines were separated on an Acquity™ UPLC column and detected with a micrOTOF-Q II mass spectrometer. The method was validated in terms of linearity, sensitivity, precision and recovery. The coefficient of determination (R 2) was higher than 0.994 for all calibration curves. The limit of detection was between 3 and 15 ng/mL. The intra- and inter-day precisions were below 5.0% and 10.7%, respectively. The proposed method was successfully applied for the biogenic amine analysis of 18 commercially available Bokbunja wines. None of the biogenic amine levels found in Bokbunja wines surpassed the toxic levels reported in the literature.
AB - A rapid and novel ultra-performance liquid chromatography/quadrupole-time of flight mass spectrometry (UPLC/Q-TOFMS) method was developed for the detection of seven biogenic amines in Korean Bokbunja wines. The biogenic amines were pre-column derivatised with dansyl chloride and subsequently extracted by a solid phase extraction cartridge. For the analysis, the dansylated amines were separated on an Acquity™ UPLC column and detected with a micrOTOF-Q II mass spectrometer. The method was validated in terms of linearity, sensitivity, precision and recovery. The coefficient of determination (R 2) was higher than 0.994 for all calibration curves. The limit of detection was between 3 and 15 ng/mL. The intra- and inter-day precisions were below 5.0% and 10.7%, respectively. The proposed method was successfully applied for the biogenic amine analysis of 18 commercially available Bokbunja wines. None of the biogenic amine levels found in Bokbunja wines surpassed the toxic levels reported in the literature.
KW - Biogenic amine
KW - Bokbunja wine
KW - Dansyl chloride
KW - Q-TOFMS
KW - UPLC
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UR - http://www.scopus.com/inward/citedby.url?scp=84856208819&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2011.11.069
DO - 10.1016/j.foodchem.2011.11.069
M3 - Article
C2 - 29243599
AN - SCOPUS:84856208819
VL - 132
SP - 1185
EP - 1190
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
IS - 3
ER -