Determination of glucan phosphorylation using heteronuclear 1H,13C double and 1H,13C, 31P triple-resonance NMR spectra

Peter Schmieder, Felix Nitschke, Martin Steup, Keven Mallow, Edgar Specker

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Phosphorylation and dephosphorylation of starch and glycogen are important for their physicochemical properties and also their physiological functions. It is therefore desirable to reliably determine the phosphorylation sites. Heteronuclear multidimensional NMR-spectroscopy is in principle a straightforward analytical approach even for complex carbohydrate molecules. With heterogeneous samples from natural sources, however, the task becomes more difficult because a full assignment of the resonances of the carbohydrates is impossible to obtain. Here, we show that the combination of heteronuclear 1H,13C and 1H,13C,31P techniques and information derived from spectra of a set of reference compounds can lead to an unambiguous determination of the phosphorylation sites even in heterogeneous samples.

Original languageEnglish (US)
Pages (from-to)655-661
Number of pages7
JournalMagnetic Resonance in Chemistry
Volume51
Issue number10
DOIs
StatePublished - Oct 1 2013

Keywords

  • heteronuclear NMR
  • phosphorylation
  • starch
  • triple resonance

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)

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