Determination of PDE-5 inhibitors and appetite suppressants in adulterated dietary supplements using LC/PDA and LC/MS

So ra Park, Jin Gyun Lee, Si Hun Roh, Gyudong Kim, Chan Hyeok Kwon, Hee Ra Park, Ki Sung Kwon, Deukjoon Kim, Sung Won Kwon

Research output: Contribution to journalArticle

17 Scopus citations


There have been a number of reports of dietary supplements contaminated with illegal adulterants that threaten consumers' health because of their adverse pharmacological effects. In the present study, a convenient and economic method was developed to detect illegal pharmaceutics, such as PDE-5 inhibitor and appetite suppressants, using liquid chromatography (LC)/photodiode array (PDA) for screening and LC/mass spectrometry (MS) for successive confirmation. Target peaks were identified by comparison of their chromatographic retention times and PDA spectra with those of synthetic standards and finally confirmed by LC/MS. As a result, tadalafil, a PDE-5 inhibitor, and N-desmethylsibutramine, a derivative of sibutramine, were detected in various dietary supplements at concentrations of 13.5-21.9mg and 3.0 mg per single dose, respectively. The present study will contribute to the development of an analytical method enabling rapid screening of a variety of health foods, and the result suggests that consumers should be aware of serious health risks related to these illegal compounds.

Original languageEnglish (US)
Pages (from-to)29-32
Number of pages4
JournalFood Additives and Contaminants: Part B Surveillance
Issue number1
Publication statusPublished - 2012



  • Dietary supplements
  • LC-PDA
  • LC/MS
  • N-desmethylsibutramine
  • Sibutramine
  • Sildenafil
  • Tadalafil

ASJC Scopus subject areas

  • Food Science
  • Public Health, Environmental and Occupational Health
  • Toxicology
  • Medicine(all)

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