Determination of protein deposits on RGP lenses by X-ray photoelectron spectroscopy

Hideji Ichijima, Tetsuji Kawai, Kazuaki Yamamoto, Harrison D Cavanagh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: To establish a novel and objective screening method for evaluating the cleaning efficacy of contact lens care solutions for removal of protein deposits on single rigid gas permeable (RGP) lenses. Methods: New and unworn RGP lenses containing no nitrogen atoms were incubated in a standard tear protein test solution. Protein deposition on lenses was determined by X-ray photoelectron spectroscopy (XPS) before and after standardized cleaning with commercially available care solutions (daily cleaners A, B) and a wetting/soaking solution or with 0.1 w/v% sodium dodecyl sulfate (SDS) as a control. Cleaning efficacy was calculated based on the percentage of nitrogen atoms present in the total elements of the measured XPS scan spectrum. Results: Nitrogen atoms originating from residual protein deposits on RGP lenses after cleaning were determined by XPS. The cleaning efficacy of the wetting/soaking solution was lower (74%) than that of daily cleaners A and B (95%) or SDS (96%) controls. Conclusions: XPS determination of residual protein deposits is a novel and direct evaluation method for determining the cleaning efficacy of RGP lens care solutions for single lenses.

Original languageEnglish (US)
Pages (from-to)18-20
Number of pages3
JournalCLAO Journal
Volume26
Issue number1
StatePublished - Jan 2000

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Photoelectron Spectroscopy
Lenses
Gases
Proteins
Nitrogen
Sodium Dodecyl Sulfate
Contact Lens Solutions

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Determination of protein deposits on RGP lenses by X-ray photoelectron spectroscopy. / Ichijima, Hideji; Kawai, Tetsuji; Yamamoto, Kazuaki; Cavanagh, Harrison D.

In: CLAO Journal, Vol. 26, No. 1, 01.2000, p. 18-20.

Research output: Contribution to journalArticle

Ichijima, Hideji ; Kawai, Tetsuji ; Yamamoto, Kazuaki ; Cavanagh, Harrison D. / Determination of protein deposits on RGP lenses by X-ray photoelectron spectroscopy. In: CLAO Journal. 2000 ; Vol. 26, No. 1. pp. 18-20.
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