Determination of proteins in infant formula by high-performance liquid chromatography-electrospray tandem mass spectrometry

Soon Sun Hong, Jeong Hill Park, Sung Won Kwon

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To determine the protein content of formula, gel electrophoresis was performed on the infant formula samples and the entire protein patterns were analyzed by nano-high performance liquid chromatography-electrospray tandem mass spectrometry (nano-HPLC/ESI/MS/MS). From the commercial infant formula profiled in this study, a total of 154 peptides, corresponding to 31 unique proteins were identified by nano-HPLC/ESI/MS/MS. Each of the identified peptides was reconfirmed by a strict integrated approach using tandem mass spectra. This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products.

Original languageEnglish (US)
Pages (from-to)69-73
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume845
Issue number1
DOIs
StatePublished - Jan 1 2007

Keywords

  • Food analysis
  • High-performance liquid chromatography
  • Infant formula
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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