Determination of secondary structure in the initiation region of ovalbumin mRNA

Charles D. Liarakos, Randolph P. Maddox, Kay A. Hilscher, Joseph R. Bishop, Darren K McGuire, Randall A. Kopper

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.

Original languageEnglish (US)
Pages (from-to)11249-11265
Number of pages17
JournalNucleic Acids Research
Volume16
Issue number23
DOIs
StatePublished - Dec 9 1988

Fingerprint

Ovalbumin
Secondary Structure
Messenger RNA
Initiator Codon
Transcription
RNA Secondary Structure
Dependent
Nucleotides
CDNA
RNA
DNA Primers
Ribonucleases
Computer program listings
Reverse
DNA
Reverse Transcription
Software
Complementary DNA

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Liarakos, C. D., Maddox, R. P., Hilscher, K. A., Bishop, J. R., McGuire, D. K., & Kopper, R. A. (1988). Determination of secondary structure in the initiation region of ovalbumin mRNA. Nucleic Acids Research, 16(23), 11249-11265. https://doi.org/10.1093/nar/16.23.11249

Determination of secondary structure in the initiation region of ovalbumin mRNA. / Liarakos, Charles D.; Maddox, Randolph P.; Hilscher, Kay A.; Bishop, Joseph R.; McGuire, Darren K; Kopper, Randall A.

In: Nucleic Acids Research, Vol. 16, No. 23, 09.12.1988, p. 11249-11265.

Research output: Contribution to journalArticle

Liarakos, CD, Maddox, RP, Hilscher, KA, Bishop, JR, McGuire, DK & Kopper, RA 1988, 'Determination of secondary structure in the initiation region of ovalbumin mRNA', Nucleic Acids Research, vol. 16, no. 23, pp. 11249-11265. https://doi.org/10.1093/nar/16.23.11249
Liarakos, Charles D. ; Maddox, Randolph P. ; Hilscher, Kay A. ; Bishop, Joseph R. ; McGuire, Darren K ; Kopper, Randall A. / Determination of secondary structure in the initiation region of ovalbumin mRNA. In: Nucleic Acids Research. 1988 ; Vol. 16, No. 23. pp. 11249-11265.
@article{d9b34aa4f5564eb18a056adb85c2c8dc,
title = "Determination of secondary structure in the initiation region of ovalbumin mRNA",
abstract = "We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.",
author = "Liarakos, {Charles D.} and Maddox, {Randolph P.} and Hilscher, {Kay A.} and Bishop, {Joseph R.} and McGuire, {Darren K} and Kopper, {Randall A.}",
year = "1988",
month = "12",
day = "9",
doi = "10.1093/nar/16.23.11249",
language = "English (US)",
volume = "16",
pages = "11249--11265",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "23",

}

TY - JOUR

T1 - Determination of secondary structure in the initiation region of ovalbumin mRNA

AU - Liarakos, Charles D.

AU - Maddox, Randolph P.

AU - Hilscher, Kay A.

AU - Bishop, Joseph R.

AU - McGuire, Darren K

AU - Kopper, Randall A.

PY - 1988/12/9

Y1 - 1988/12/9

N2 - We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.

AB - We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.

UR - http://www.scopus.com/inward/record.url?scp=0024273505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024273505&partnerID=8YFLogxK

U2 - 10.1093/nar/16.23.11249

DO - 10.1093/nar/16.23.11249

M3 - Article

C2 - 3205742

AN - SCOPUS:0024273505

VL - 16

SP - 11249

EP - 11265

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 23

ER -