Determination of secondary structure in the initiation region of ovalbumin mRNA

Charles D. Liarakos, Randolph P. Maddox, Kay A. Hilscher, Joseph R. Bishop, Darren K McGuire, Randall A. Kopper

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.

Original languageEnglish (US)
Pages (from-to)11249-11265
Number of pages17
JournalNucleic acids research
Volume16
Issue number23
DOIs
StatePublished - Dec 9 1988

ASJC Scopus subject areas

  • Genetics

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