Determination of the intracellular sodium concentration in perfused mouse liver by 31P and 23Na magnetic resonance spectroscopy

Jean Marie Colet, Janice D. Makos, Craig R. Malloy, A. Dean Sherry

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A combination of 31P and 23Na NMR spectroscopy has been used to quantify the concentration of intracellular sodium, [Na](IC) in the isolated and perfused mouse liver. The 31P resonances of dimethyl methylphosphonate and LaDOTP6-, markers of total tissue space and extracellular space, respectively, were used to determine the intracellular liver volume. For a mean wet weight of 1.7 ± 0.3 g, the intracellular liver volume as measured by 31P NMR averaged 1.2 ± 0.2 ml. The amount of intracellular sodium was measured from the baseline-resolved intracellular 23Na resonance during perfusion of the shift reagent, TmDOTP5-. These two measurements resulted in an NMR-determined value for [Na](IC) of 29.0 ± 5.2 mM. Separate measurement of total tissue Tm and Na by atomic absorption spectroscopy on the same samples provided an AAS-determined value for [Na](IC) of 32.1 ± 7.4 mM. These results indicate that intracellular sodium in the isolated, perfused liver is 100% visible by 23Na NMR spectroscopy.

Original languageEnglish (US)
Pages (from-to)155-159
Number of pages5
JournalMagnetic resonance in medicine
Volume39
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • Liver
  • NMR
  • Phosphorus space markers
  • Shift reagent

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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