High performance liquid chromatography-ultraviolet (HPLC-UV) detection method was performed to simultaneously determine three preservatives: cresol, chlorocresol and benzethonium. According to the current Korean Pharmacopeia Codex, the only-available analytical method for discerning the three preservatives uses thin-layer chromatography, which does not provide accurate detection or quantification. Therefore, this study focused on developing a new HPLC-UV method which gives fast, accurate and sensitive outcomes. The experiment was done using Phenomenex Gemini column [C18 (250 × 4.6 mm, 5 μm)] and the mobile system was methanol and 0.01 M potassium phosphate (monobasic) in water (pH = 3.3). The developed method was validated through linearity, range, system suitability, precision, accuracy, recovery and inter-laboratory variation.
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
- Pharmaceutical Science
Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection. / Lee, Jin Gyun; Shin, Sang yeon; Shin, Hae jin; Huh, Yoonyoung; Lee, Seul Ji; Kim, Dong Hwan; Lee, Sujung; Kim, Young Ok; Han, Sang Beom; Lee, Jeongmi; Park, Jeong Hill; Kwon, Sung Won.In: Journal of Pharmaceutical Investigation, Vol. 42, No. 1, 02.2012, p. 47-50.
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