Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection

Jin Gyun Lee, Sang yeon Shin, Hae jin Shin, Yoonyoung Huh, Seul Ji Lee, Dong Hwan Kim, Sujung Lee, Young Ok Kim, Sang Beom Han, Jeongmi Lee, Jeong Hill Park, Sung Won Kwon

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

High performance liquid chromatography-ultraviolet (HPLC-UV) detection method was performed to simultaneously determine three preservatives: cresol, chlorocresol and benzethonium. According to the current Korean Pharmacopeia Codex, the only-available analytical method for discerning the three preservatives uses thin-layer chromatography, which does not provide accurate detection or quantification. Therefore, this study focused on developing a new HPLC-UV method which gives fast, accurate and sensitive outcomes. The experiment was done using Phenomenex Gemini column [C18 (250 × 4.6 mm, 5 μm)] and the mobile system was methanol and 0.01 M potassium phosphate (monobasic) in water (pH = 3.3). The developed method was validated through linearity, range, system suitability, precision, accuracy, recovery and inter-laboratory variation.

Original languageEnglish (US)
Pages (from-to)47-50
Number of pages4
JournalJournal of Pharmaceutical Investigation
Volume42
Issue number1
DOIs
StatePublished - Feb 1 2012

Keywords

  • Benzethonium
  • Chlorocresol
  • Cresol
  • HPLC-UV
  • Preservatives

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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    Lee, J. G., Shin, S. Y., Shin, H. J., Huh, Y., Lee, S. J., Kim, D. H., Lee, S., Kim, Y. O., Han, S. B., Lee, J., Park, J. H., & Kwon, S. W. (2012). Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection. Journal of Pharmaceutical Investigation, 42(1), 47-50. https://doi.org/10.1007/s40005-012-0008-5