Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection

Jin Gyun Lee; Sang yeon Shin; Hae jin Shin; Yoonyoung Huh; Seul Ji Lee; Dong Hwan Kim; Sujung Lee; Young Ok Kim; Sang Beom Han; Jeongmi Lee; Jeong Hill Park; Sung Won Kwon

doi:10.1007/s40005-012-0008-5

Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection

Jin Gyun Lee, Sang yeon Shin, Hae jin Shin, Yoonyoung Huh, Seul Ji Lee, Dong Hwan Kim, Sujung Lee, Young Ok Kim, Sang Beom Han, Jeongmi Lee, Jeong Hill Park, Sung Won Kwon

Physiology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

High performance liquid chromatography-ultraviolet (HPLC-UV) detection method was performed to simultaneously determine three preservatives: cresol, chlorocresol and benzethonium. According to the current Korean Pharmacopeia Codex, the only-available analytical method for discerning the three preservatives uses thin-layer chromatography, which does not provide accurate detection or quantification. Therefore, this study focused on developing a new HPLC-UV method which gives fast, accurate and sensitive outcomes. The experiment was done using Phenomenex Gemini column [C18 (250 × 4.6 mm, 5 μm)] and the mobile system was methanol and 0.01 M potassium phosphate (monobasic) in water (pH = 3.3). The developed method was validated through linearity, range, system suitability, precision, accuracy, recovery and inter-laboratory variation.

Original language	English (US)
Pages (from-to)	47-50
Number of pages	4
Journal	Journal of Pharmaceutical Investigation
Volume	42
Issue number	1
DOIs	https://doi.org/10.1007/s40005-012-0008-5
State	Published - Feb 2012

Keywords

Benzethonium
Chlorocresol
Cresol
HPLC-UV
Preservatives

ASJC Scopus subject areas

Pharmaceutical Science
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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10.1007/s40005-012-0008-5

Cite this

Lee, J. G., Shin, S. Y., Shin, H. J., Huh, Y., Lee, S. J., Kim, D. H., Lee, S., Kim, Y. O., Han, S. B., Lee, J., Park, J. H., & Kwon, S. W. (2012). Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection. Journal of Pharmaceutical Investigation, 42(1), 47-50. https://doi.org/10.1007/s40005-012-0008-5

Lee, JG, Shin, SY, Shin, HJ, Huh, Y, Lee, SJ, Kim, DH, Lee, S, Kim, YO, Han, SB, Lee, J, Park, JH & Kwon, SW 2012, 'Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection', Journal of Pharmaceutical Investigation, vol. 42, no. 1, pp. 47-50. https://doi.org/10.1007/s40005-012-0008-5

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title = "Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection",

abstract = "High performance liquid chromatography-ultraviolet (HPLC-UV) detection method was performed to simultaneously determine three preservatives: cresol, chlorocresol and benzethonium. According to the current Korean Pharmacopeia Codex, the only-available analytical method for discerning the three preservatives uses thin-layer chromatography, which does not provide accurate detection or quantification. Therefore, this study focused on developing a new HPLC-UV method which gives fast, accurate and sensitive outcomes. The experiment was done using Phenomenex Gemini column [C18 (250 × 4.6 mm, 5 μm)] and the mobile system was methanol and 0.01 M potassium phosphate (monobasic) in water (pH = 3.3). The developed method was validated through linearity, range, system suitability, precision, accuracy, recovery and inter-laboratory variation.",

keywords = "Benzethonium, Chlorocresol, Cresol, HPLC-UV, Preservatives",

author = "Lee, {Jin Gyun} and Shin, {Sang yeon} and Shin, {Hae jin} and Yoonyoung Huh and Lee, {Seul Ji} and Kim, {Dong Hwan} and Sujung Lee and Kim, {Young Ok} and Han, {Sang Beom} and Jeongmi Lee and Park, {Jeong Hill} and Kwon, {Sung Won}",

note = "Funding Information: Acknowledgment This work was supported by a grant (09102 KFDA301) from the Korean Food and Drug Administration in 2009.",

year = "2012",

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doi = "10.1007/s40005-012-0008-5",

language = "English (US)",

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AU - Lee, Jin Gyun

AU - Shin, Sang yeon

AU - Shin, Hae jin

AU - Huh, Yoonyoung

AU - Lee, Seul Ji

AU - Kim, Dong Hwan

AU - Lee, Sujung

AU - Kim, Young Ok

AU - Han, Sang Beom

AU - Lee, Jeongmi

AU - Park, Jeong Hill

AU - Kwon, Sung Won

N1 - Funding Information: Acknowledgment This work was supported by a grant (09102 KFDA301) from the Korean Food and Drug Administration in 2009.

PY - 2012/2

Y1 - 2012/2

N2 - High performance liquid chromatography-ultraviolet (HPLC-UV) detection method was performed to simultaneously determine three preservatives: cresol, chlorocresol and benzethonium. According to the current Korean Pharmacopeia Codex, the only-available analytical method for discerning the three preservatives uses thin-layer chromatography, which does not provide accurate detection or quantification. Therefore, this study focused on developing a new HPLC-UV method which gives fast, accurate and sensitive outcomes. The experiment was done using Phenomenex Gemini column [C18 (250 × 4.6 mm, 5 μm)] and the mobile system was methanol and 0.01 M potassium phosphate (monobasic) in water (pH = 3.3). The developed method was validated through linearity, range, system suitability, precision, accuracy, recovery and inter-laboratory variation.

AB - High performance liquid chromatography-ultraviolet (HPLC-UV) detection method was performed to simultaneously determine three preservatives: cresol, chlorocresol and benzethonium. According to the current Korean Pharmacopeia Codex, the only-available analytical method for discerning the three preservatives uses thin-layer chromatography, which does not provide accurate detection or quantification. Therefore, this study focused on developing a new HPLC-UV method which gives fast, accurate and sensitive outcomes. The experiment was done using Phenomenex Gemini column [C18 (250 × 4.6 mm, 5 μm)] and the mobile system was methanol and 0.01 M potassium phosphate (monobasic) in water (pH = 3.3). The developed method was validated through linearity, range, system suitability, precision, accuracy, recovery and inter-laboratory variation.

KW - Benzethonium

KW - Chlorocresol

KW - Cresol

KW - HPLC-UV

KW - Preservatives

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Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detection

Abstract

Keywords

ASJC Scopus subject areas

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