Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection

H. Liu, L. J. Forman, J. Montoya, C. Eggers, C. Barham, M. Delgado

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The derivatization of valproic acid and undecylenic acid with 4-bromomethyl-7-methoxycoumarin is described. The derivatives were detected by photodiode-array and fluorescence detectors. The optimum monitoring conditions and the stability of the suspension solution and derivatives were investigated. A high-performance liquid chromatographic (HPLC) method with isocratic or gradient elution has been established for the analysis. This method has been used for the determination of total and free valproic acid in serum. There is a satisfactory correlation between the results obtained by this HPLC method and those measured by the enzyme immunoassay. Some other common anti-epileptic drugs did not interfere with the analysis. The method is simple and fast and has better sensitivity and linearity than enzyme immunoassay. It is suited for routine therapeutic drug monitoring.

Original languageEnglish (US)
Pages (from-to)163-169
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume576
Issue number1
DOIs
StatePublished - Apr 15 1992

Fingerprint

Valproic Acid
High performance liquid chromatography
Photodiodes
Fluorescence
High Pressure Liquid Chromatography
Derivatives
Liquids
Condition monitoring
Enzymes
Immunoenzyme Techniques
Pharmaceutical Preparations
Suspensions
Detectors
Monitoring
Drug Monitoring
Serum
undecylenic acid
4-bromomethyl-7-methoxycoumarin

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection. / Liu, H.; Forman, L. J.; Montoya, J.; Eggers, C.; Barham, C.; Delgado, M.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 576, No. 1, 15.04.1992, p. 163-169.

Research output: Contribution to journalArticle

@article{39c3f78cb0314b74aaae0cd1b1dd7adf,
title = "Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection",
abstract = "The derivatization of valproic acid and undecylenic acid with 4-bromomethyl-7-methoxycoumarin is described. The derivatives were detected by photodiode-array and fluorescence detectors. The optimum monitoring conditions and the stability of the suspension solution and derivatives were investigated. A high-performance liquid chromatographic (HPLC) method with isocratic or gradient elution has been established for the analysis. This method has been used for the determination of total and free valproic acid in serum. There is a satisfactory correlation between the results obtained by this HPLC method and those measured by the enzyme immunoassay. Some other common anti-epileptic drugs did not interfere with the analysis. The method is simple and fast and has better sensitivity and linearity than enzyme immunoassay. It is suited for routine therapeutic drug monitoring.",
author = "H. Liu and Forman, {L. J.} and J. Montoya and C. Eggers and C. Barham and M. Delgado",
year = "1992",
month = "4",
day = "15",
doi = "10.1016/0378-4347(92)80189-W",
language = "English (US)",
volume = "576",
pages = "163--169",
journal = "Journal of Chromatography - Biomedical Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection

AU - Liu, H.

AU - Forman, L. J.

AU - Montoya, J.

AU - Eggers, C.

AU - Barham, C.

AU - Delgado, M.

PY - 1992/4/15

Y1 - 1992/4/15

N2 - The derivatization of valproic acid and undecylenic acid with 4-bromomethyl-7-methoxycoumarin is described. The derivatives were detected by photodiode-array and fluorescence detectors. The optimum monitoring conditions and the stability of the suspension solution and derivatives were investigated. A high-performance liquid chromatographic (HPLC) method with isocratic or gradient elution has been established for the analysis. This method has been used for the determination of total and free valproic acid in serum. There is a satisfactory correlation between the results obtained by this HPLC method and those measured by the enzyme immunoassay. Some other common anti-epileptic drugs did not interfere with the analysis. The method is simple and fast and has better sensitivity and linearity than enzyme immunoassay. It is suited for routine therapeutic drug monitoring.

AB - The derivatization of valproic acid and undecylenic acid with 4-bromomethyl-7-methoxycoumarin is described. The derivatives were detected by photodiode-array and fluorescence detectors. The optimum monitoring conditions and the stability of the suspension solution and derivatives were investigated. A high-performance liquid chromatographic (HPLC) method with isocratic or gradient elution has been established for the analysis. This method has been used for the determination of total and free valproic acid in serum. There is a satisfactory correlation between the results obtained by this HPLC method and those measured by the enzyme immunoassay. Some other common anti-epileptic drugs did not interfere with the analysis. The method is simple and fast and has better sensitivity and linearity than enzyme immunoassay. It is suited for routine therapeutic drug monitoring.

UR - http://www.scopus.com/inward/record.url?scp=0026606246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026606246&partnerID=8YFLogxK

U2 - 10.1016/0378-4347(92)80189-W

DO - 10.1016/0378-4347(92)80189-W

M3 - Article

C2 - 1500452

AN - SCOPUS:0026606246

VL - 576

SP - 163

EP - 169

JO - Journal of Chromatography - Biomedical Applications

JF - Journal of Chromatography - Biomedical Applications

SN - 0378-4347

IS - 1

ER -