Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection

H. Liu; L. J. Forman; J. Montoya; C. Eggers; C. Barham; M. Delgado

doi:10.1016/0378-4347(92)80189-W

Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection

H. Liu, L. J. Forman, J. Montoya, C. Eggers, C. Barham, M. Delgado

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Abstract

The derivatization of valproic acid and undecylenic acid with 4-bromomethyl-7-methoxycoumarin is described. The derivatives were detected by photodiode-array and fluorescence detectors. The optimum monitoring conditions and the stability of the suspension solution and derivatives were investigated. A high-performance liquid chromatographic (HPLC) method with isocratic or gradient elution has been established for the analysis. This method has been used for the determination of total and free valproic acid in serum. There is a satisfactory correlation between the results obtained by this HPLC method and those measured by the enzyme immunoassay. Some other common anti-epileptic drugs did not interfere with the analysis. The method is simple and fast and has better sensitivity and linearity than enzyme immunoassay. It is suited for routine therapeutic drug monitoring.

Original language	English (US)
Pages (from-to)	163-169
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	576
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(92)80189-W
State	Published - Apr 15 1992

ASJC Scopus subject areas

General Chemistry

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10.1016/0378-4347(92)80189-W

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title = "Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection",

abstract = "The derivatization of valproic acid and undecylenic acid with 4-bromomethyl-7-methoxycoumarin is described. The derivatives were detected by photodiode-array and fluorescence detectors. The optimum monitoring conditions and the stability of the suspension solution and derivatives were investigated. A high-performance liquid chromatographic (HPLC) method with isocratic or gradient elution has been established for the analysis. This method has been used for the determination of total and free valproic acid in serum. There is a satisfactory correlation between the results obtained by this HPLC method and those measured by the enzyme immunoassay. Some other common anti-epileptic drugs did not interfere with the analysis. The method is simple and fast and has better sensitivity and linearity than enzyme immunoassay. It is suited for routine therapeutic drug monitoring.",

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T1 - Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection

AU - Liu, H.

AU - Forman, L. J.

AU - Montoya, J.

AU - Eggers, C.

AU - Barham, C.

AU - Delgado, M.

PY - 1992/4/15

Y1 - 1992/4/15

N2 - The derivatization of valproic acid and undecylenic acid with 4-bromomethyl-7-methoxycoumarin is described. The derivatives were detected by photodiode-array and fluorescence detectors. The optimum monitoring conditions and the stability of the suspension solution and derivatives were investigated. A high-performance liquid chromatographic (HPLC) method with isocratic or gradient elution has been established for the analysis. This method has been used for the determination of total and free valproic acid in serum. There is a satisfactory correlation between the results obtained by this HPLC method and those measured by the enzyme immunoassay. Some other common anti-epileptic drugs did not interfere with the analysis. The method is simple and fast and has better sensitivity and linearity than enzyme immunoassay. It is suited for routine therapeutic drug monitoring.

AB - The derivatization of valproic acid and undecylenic acid with 4-bromomethyl-7-methoxycoumarin is described. The derivatives were detected by photodiode-array and fluorescence detectors. The optimum monitoring conditions and the stability of the suspension solution and derivatives were investigated. A high-performance liquid chromatographic (HPLC) method with isocratic or gradient elution has been established for the analysis. This method has been used for the determination of total and free valproic acid in serum. There is a satisfactory correlation between the results obtained by this HPLC method and those measured by the enzyme immunoassay. Some other common anti-epileptic drugs did not interfere with the analysis. The method is simple and fast and has better sensitivity and linearity than enzyme immunoassay. It is suited for routine therapeutic drug monitoring.

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Determination of valproic acid by high-performance liquid chromatography with photodiode-array and fluorescence detection

Abstract

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