TY - JOUR
T1 - Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue
AU - Siebelmann, Sebastian
AU - Gehlsen, Uta
AU - Hüttmann, Gereon
AU - Koop, Norbert
AU - Bölke, Torsten
AU - Gebert, Andreas
AU - Stern, Michael E.
AU - Niederkorn, Jerry Y.
AU - Steven, Philipp
PY - 2013/12/20
Y1 - 2013/12/20
N2 - Purpose: Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model. Methods: Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy. Results: Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 μm/min. Conclusions: CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration. Copyright:
AB - Purpose: Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model. Methods: Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy. Results: Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 μm/min. Conclusions: CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration. Copyright:
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U2 - 10.1371/journal.pone.0082355
DO - 10.1371/journal.pone.0082355
M3 - Article
C2 - 24376530
AN - SCOPUS:84893378259
SN - 1932-6203
VL - 8
JO - PloS one
JF - PloS one
IS - 12
M1 - e82355
ER -