Development of 3D Lymph Node Mimetic for Studying Prostate Cancer Metastasis

Amirhossein Hakamivala, Yi Hui Huang, Yung Fu Chang, Zui Pan, Ashwin Nair, Jer Tsong Hsieh, Liping Tang

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Lymph node (LN) metastasis causes poor prognosis for patients with prostate cancer (PCa). Although LN-cells and cellular responses play a pivotal role in cancer metastasis, the interplay between LN-cells and PCa cells is undetermined due to the small size and widespread distribution of LNs. To identify factors responsible for LN metastasis, a 3D cell culture biosystem is fabricated to simulate LN responses during metastasis. First, it is determined that LN explants previously exposed to high metastatic PCa release substantially more chemotactic factors to promote metastatic PCa migration than those exposed to low-metastatic PCa. Furthermore, T-lymphocytes are found to produce chemotactic factors in LNs, among which, CXCL12, CCL21, and IL-10 are identified to have the most chemotactic effect. To mimic the LN microenvironment, Cytodex beads are seeded with T cells to produce a LN-mimetic biosystem in both static and flow conditions. As expected, the flow condition permits prolonged cellular responses. Interestingly, when PCa cells with varying metastatic potentials are introduced into the system, it produces PCa-specific chemokines accordingly. These results support that the LN mimetic helps in analyzing the processes underlying metastasized LNs and for testing various treatments to reduce cancer LN metastasis.

Original languageEnglish (US)
Article number1900019
JournalAdvanced Biosystems
Volume3
Issue number9
DOIs
StatePublished - Sep 1 2019

Keywords

  • T lymphocytes
  • lymph node biosystem
  • lymph nodes
  • metastasis
  • prostate cancer

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Hakamivala, A., Huang, Y. H., Chang, Y. F., Pan, Z., Nair, A., Hsieh, J. T., & Tang, L. (2019). Development of 3D Lymph Node Mimetic for Studying Prostate Cancer Metastasis. Advanced Biosystems, 3(9), [1900019]. https://doi.org/10.1002/adbi.201900019