Development of a new polarized hyperspectral imaging microscope

Ximing Zhou, Ling Ma, Martin Halicek, James Dormer, Baowei Fei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we proposed and designed a transmission mode polarized hyperspectral imaging microscope (PHSIM). The hyperspectral imaging (HSI) component is based on the snapscan with a hyperspectral camera. The HSI wavelength range is from 467-700 nm. Polarized light imaging is realized by the integration of two polarizers and two liquid crystal variable retarders (LCVR), which is capable of full Stokes polarimetric imaging. The new imaging device was tested for the detection of squamous cell carcinoma (SCC) in H&E stained oral tissue slides of 8 patients. One normal area and one cancerous area on each slide are selected to make the comparison. The preliminary results indicated that the spectral curves of the Stokes vector parameters (S0, S1, S2, S3) of the normal area on the H&E stained oral tissue slides are different from those of SCC in certain wavelength range. Further work is required to apply the new polarized hyperspectral imaging microscope to a large number of patient samples and to test the PHSIM system in different cancer types.

Original languageEnglish (US)
Title of host publicationImaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2020
EditorsBrian Jet-Fei Wong, Justus F. Ilgner
PublisherSPIE
ISBN (Electronic)9781510631892
DOIs
StatePublished - 2020
EventImaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2020 - San Francisco, United States
Duration: Feb 1 2020 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11213
ISSN (Print)1605-7422

Conference

ConferenceImaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2020
CountryUnited States
CitySan Francisco
Period2/1/20 → …

Keywords

  • Head and neck cancer
  • Hyperspectral imaging
  • Machine learning
  • Polarized light imaging
  • Stokes vector

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Zhou, X., Ma, L., Halicek, M., Dormer, J., & Fei, B. (2020). Development of a new polarized hyperspectral imaging microscope. In B. J-F. Wong, & J. F. Ilgner (Eds.), Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2020 [1121308] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11213). SPIE. https://doi.org/10.1117/12.2549676