Development of high performance liquid chromatography-ultraviolet detection method for screening mebendazole, clorsulon, diaveridine, and tolfenamic acid in animal-based food samples

Yun Pyo Kang, Jin Yu, Yoonyoung Huh, Jae Ho Oh, Chan Hyeok Kwon, Seul Ji Lee, Ji Won Ee, Geun Tae Kim, Jin Gyun Lee, Jeongmi Lee, Jeong Hill Park, You Sun Kim, Sung Won Kwon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study focused on the detection and validation of the residues of the four veterinary drugs, mebendazole, clorsulon, diaveridine, and tolfenamic acid, using high performance liquid chromatography (HPLC) and an ultraviolet (UV) detector. Utilizing C18 column as a stationary phase and applying appropriate mobile phases to each analysis according to the properties of the analytes, target compounds in food samples were successfully detected and separated within 15-50 min. Additionally, in order to optimize detection, liquid-liquid extraction (LLE) and purification steps were established to minimize the endogenous peaks and their interferences. The method was validated through testing of linearity, accuracy, precision, the limit of detection (LOD) and the limit of quantification (LOQ). The LOQ levels of the four drugs were lower than the maximum residual limit, and the coefficient of determination (R2) was over 0.99. The recovery results ranged from 82.3-105.2%, 79.3-83.3%, 79.4-86.0%, and 81.7-88.5% with relative standard deviations lower than 20% for mebendazole, clorsulon, diaveridine, and tolfenamic acid, respectively, corresponding to the CODEX guideline. This proposed method reduces costs and enables easier application in rural or remote areas where testing facilities or instruments often are unavailable.

Original languageEnglish (US)
Pages (from-to)246-256
Number of pages11
JournalDrug Testing and Analysis
Volume6
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Mebendazole
High performance liquid chromatography
detection method
liquid chromatography
Screening
Animals
High Pressure Liquid Chromatography
Veterinary Drugs
Ultraviolet detectors
Food
Liquid-Liquid Extraction
food
animal
acid
Liquids
Testing
drug
Purification
Limit of Detection
liquid

Keywords

  • Clorsulon
  • Diaveridine
  • HPLC-UV
  • Mebendazole
  • Tolfenamic acid; veterinary drugs

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Pharmaceutical Science
  • Spectroscopy

Cite this

Development of high performance liquid chromatography-ultraviolet detection method for screening mebendazole, clorsulon, diaveridine, and tolfenamic acid in animal-based food samples. / Kang, Yun Pyo; Yu, Jin; Huh, Yoonyoung; Oh, Jae Ho; Kwon, Chan Hyeok; Lee, Seul Ji; Ee, Ji Won; Kim, Geun Tae; Lee, Jin Gyun; Lee, Jeongmi; Park, Jeong Hill; Kim, You Sun; Kwon, Sung Won.

In: Drug Testing and Analysis, Vol. 6, No. 3, 2014, p. 246-256.

Research output: Contribution to journalArticle

Kang, Yun Pyo ; Yu, Jin ; Huh, Yoonyoung ; Oh, Jae Ho ; Kwon, Chan Hyeok ; Lee, Seul Ji ; Ee, Ji Won ; Kim, Geun Tae ; Lee, Jin Gyun ; Lee, Jeongmi ; Park, Jeong Hill ; Kim, You Sun ; Kwon, Sung Won. / Development of high performance liquid chromatography-ultraviolet detection method for screening mebendazole, clorsulon, diaveridine, and tolfenamic acid in animal-based food samples. In: Drug Testing and Analysis. 2014 ; Vol. 6, No. 3. pp. 246-256.
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AU - Huh, Yoonyoung

AU - Oh, Jae Ho

AU - Kwon, Chan Hyeok

AU - Lee, Seul Ji

AU - Ee, Ji Won

AU - Kim, Geun Tae

AU - Lee, Jin Gyun

AU - Lee, Jeongmi

AU - Park, Jeong Hill

AU - Kim, You Sun

AU - Kwon, Sung Won

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