Development of increased cytoplasmic binding of androgen in the submandibular gland of the mouse with testicular feminization

J. F. Dunn, J. L. Goldstein, J. D. Wilson

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

A new catabolite with a 2 carbon shorter side chain and a trans ureido ring, d allobisnorbiotin, was isolated from the culture filtrates of a pseudomonad grown on double labeled d biotin. Most of the original ureido carbonyl 14C of biotin was lost but 3H in the thiolane ring and the side chain portion is retained in the catabolite. Maximal amounts of d allobisnorbiotin are formed under conditions for bacterial growth where aeration is limited. Formation of this catabolite must occur from the level of bisnorbiotin by way of cleavage of the cis ureido ring at the bridgehead carbon opposite the side chain, followed by decarbamylation and recarbamylation to form the trans isomer.

Original languageEnglish (US)
Pages (from-to)7819-7825
Number of pages7
JournalJournal of Biological Chemistry
Volume248
Issue number22
StatePublished - Jan 1 1973

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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