Development of multipurpose peroxisomes in Candida boidinii grown in oleic acid-methanol limited continuous cultures

H. R. Waterham, I. Keizer-Gunnink, J. M. Goodman, W. Harder, M. Veenhuis

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Abstract

We have studied the development and metabolic significance of peroxisomes in the yeast Candida boidinii following adaptation of the organism to cultivation conditions which require the simultaneous presence and activity of two independent peroxisome-mediated pathways for growth. After the addition of methanol to oleic acid-grown cells at late exponential growth, a number of new small peroxisomes developed which, apart from the presence of β-oxidation enzymes, were characterized by the presence of enzymes involved in methanol metabolism (alcohol oxidase and dihydroxyacetone synthase). The latter proteins, however, were absent in the larger organelles which were originally present in the oleic acid-grown cells prior to the addition of methanol and which contained only enzymes of the β-oxidation pathway. Subsequent experiments on cells from continuous cultures grown on a mixture of oleic acid and methanol at steady-state conditions revealed that both the enzymes of the β-oxidation pathway and those involved in methanol metabolism were found in one and the same compartment. Thus, under these conditions the cells contained peroxisomes which were concurrently involved in the metabolism of two different carbon sources simultaneously used for growth. Our results indicated that the heterogeneity in the peroxisomal population of a single cell, observed in the transient state following the addition of methanol, is only temporary and due to heterogeneity among these organelles with respect to their capacity to incorporate newly synthesized matrix proteins.

Original languageEnglish (US)
Pages (from-to)4057-4063
Number of pages7
JournalJournal of Bacteriology
Volume174
Issue number12
StatePublished - 1992

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Peroxisomes
Oleic Acid
Candida
Methanol
formaldehyde transketolase
Enzymes
alcohol oxidase
Organelles
Growth
Proteins
Carbon
Cell Culture Techniques
Yeasts
Population

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Development of multipurpose peroxisomes in Candida boidinii grown in oleic acid-methanol limited continuous cultures. / Waterham, H. R.; Keizer-Gunnink, I.; Goodman, J. M.; Harder, W.; Veenhuis, M.

In: Journal of Bacteriology, Vol. 174, No. 12, 1992, p. 4057-4063.

Research output: Contribution to journalArticle

Waterham, H. R. ; Keizer-Gunnink, I. ; Goodman, J. M. ; Harder, W. ; Veenhuis, M. / Development of multipurpose peroxisomes in Candida boidinii grown in oleic acid-methanol limited continuous cultures. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 12. pp. 4057-4063.
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