Development of outer hair cells in Ames waltzer mice: Mutation in protocadherin 15 affects development of cuticular plate and associated structures

Yayoi S. Kikkawa, Karen S. Pawlowski, Charles G. Wright, Kumar N. Alagramam

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Ames waltzer (av) mouse mutant harbors a mutation in the protocadherin 15 gene (Pcdh15) and is a model for deafness in Usher syndrome 1F and nonsyndromic deafness DFNB23. Mutation in Pcdh15 affects stereocilia morphogenesis and polarity. Disruptions of apical cellular components in outer hair cells have also been described in av mutants. Organization of stereocilia and cell polarization may be dependent on proper orientation of structural components residing in the apical portion of the cell during development. We used electron and immunofluorescent microscopy to examine structural maturation of outer hair cells in av3J mice with emphasis on the fonticulus, basal body/ centriole complex, actin mesh, and the microtubule network during initiation of bundle organization, between embryonic day (E) 16.5 and postnatal day 5 (P5). We found major ultrastructural rearrangements near the hair cell surface in av3J mice. Earliest changes were in kinocilia, basal body, and stereocilia positioning and microtubule arrangement once the kinocilia had lateralized to the side of the cell (between E16.5 and postnatal day [P] 0, before cuticular plate formation and stereocilia elongation). By P0, the developing fonticulus in av mice appeared enlarged, with a normal vesicle density. Stereocilia bundle disorganization increased after P0, with disruptions of the actin mesh within the cuticular plate. These observations support the hypothesis that mutations in Pcdh15 in av3J mice adversely affect coordinated maturation of apical cell components, resulting in disturbed stereocilia bundle polarity in av mice.

Original languageEnglish (US)
Pages (from-to)224-232
Number of pages9
JournalAnatomical Record
Volume291
Issue number2
DOIs
StatePublished - 2008

Fingerprint

Outer Auditory Hair Cells
Stereocilia
cadherins
hair
hairs
mutation
Mutation
gene
maturation
mice
Basal Bodies
structural component
deafness
morphogenesis
cells
vesicle
Microtubules
positioning
Actins
microscopy

Keywords

  • Ames waltzer
  • Basal body/centriole
  • Fonticulus
  • Kinocilia
  • Polarity
  • Protocadherin 15 (Pcdh15)
  • Stereocilia

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology

Cite this

Development of outer hair cells in Ames waltzer mice : Mutation in protocadherin 15 affects development of cuticular plate and associated structures. / Kikkawa, Yayoi S.; Pawlowski, Karen S.; Wright, Charles G.; Alagramam, Kumar N.

In: Anatomical Record, Vol. 291, No. 2, 2008, p. 224-232.

Research output: Contribution to journalArticle

Kikkawa, Yayoi S. ; Pawlowski, Karen S. ; Wright, Charles G. ; Alagramam, Kumar N. / Development of outer hair cells in Ames waltzer mice : Mutation in protocadherin 15 affects development of cuticular plate and associated structures. In: Anatomical Record. 2008 ; Vol. 291, No. 2. pp. 224-232.
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