Developmental changes in steroidogenic enzymes in human postnatal adrenal cortex: Immunohistochemical studies

Takashi Suzuki, Hironobu Sasano, Junji Takeyama, Chika Kaneko, William A. Freije, Bruce R. Carr, William E. Rainey

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

OBJECTIVE: Adrenarche is considered to occur as a result of intra-adrenal changes in steroidogenic enzymes involved in C19 steroid production. The present study was conducted because developmental changes in steroidogenic enzymes have not been examined well in human postnatal adrenal. PATIENTS AND MEASUREMENTS: Twenty-four specimens of nonpathological human adrenals from 7 months to 62 years retrieved from autopsy files. Immunohistochemistry for P450 side-chain cleavage (P450scc), 17α hydroxylase (P450c17), dehydroepiandrosterone sulfotransferase (DHEA-ST), P450 oxidoreductase, cytochrome b5, and 3β-hydroxysteroid dehydrogenase (3βHSD) was performed in these specimens, and the immunointensity was evaluated using CAS 200 computed image analysis system. RESULTS: Immunoreactivity of P450scc was marked in the zona glomerulosa, fasciculata and reticularis in the adrenal glands of all the cases examined. P450c17 and DHEA-ST immunoreactivity was weak in the zona fasciculata and reticularis in the adrenals of age 7 months to 5 years, but thereafter became prominent in the zona reticularis. Immunoreactivity of P450 oxidoreductase and cytochrome b5, components of the electron transfer system hypothesized to regulate the 17-20 lyase activity of P450c17, was weak in all three zones of adrenal cortex from 7 months to 5 years, and became more marked in the zona reticularis after age 5 years. 3βHSD immunoreactivity was marked in all three zones of the adrenal cortex from 7 months to 8 years but thereafter decreased in the zona reticularis. CONCLUSIONS: These data suggest that the human adrenal zona reticularis markedly begins to develop morphologically and functionally at around 5 years of age. The increased level of P450c17, DHEA-ST, P450 oxidoreductase, and cytochrome b5, and the decreased level of 3βHSD in the reticularis is likely to contribute to increased C19 steroid production during adrenarche.

Original languageEnglish (US)
Pages (from-to)739-747
Number of pages9
JournalClinical Endocrinology
Volume53
Issue number6
DOIs
StatePublished - 2000

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Zona Reticularis
Adrenal Cortex
3-Hydroxysteroid Dehydrogenases
Mixed Function Oxygenases
Cytochromes b5
Enzymes
Adrenarche
Zona Fasciculata
Oxidoreductases
Steroids
Steroid 17-alpha-Hydroxylase
Zona Glomerulosa
Adrenal Glands
Autopsy
Immunohistochemistry
Electrons

ASJC Scopus subject areas

  • Endocrinology

Cite this

Developmental changes in steroidogenic enzymes in human postnatal adrenal cortex : Immunohistochemical studies. / Suzuki, Takashi; Sasano, Hironobu; Takeyama, Junji; Kaneko, Chika; Freije, William A.; Carr, Bruce R.; Rainey, William E.

In: Clinical Endocrinology, Vol. 53, No. 6, 2000, p. 739-747.

Research output: Contribution to journalArticle

Suzuki, Takashi ; Sasano, Hironobu ; Takeyama, Junji ; Kaneko, Chika ; Freije, William A. ; Carr, Bruce R. ; Rainey, William E. / Developmental changes in steroidogenic enzymes in human postnatal adrenal cortex : Immunohistochemical studies. In: Clinical Endocrinology. 2000 ; Vol. 53, No. 6. pp. 739-747.
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abstract = "OBJECTIVE: Adrenarche is considered to occur as a result of intra-adrenal changes in steroidogenic enzymes involved in C19 steroid production. The present study was conducted because developmental changes in steroidogenic enzymes have not been examined well in human postnatal adrenal. PATIENTS AND MEASUREMENTS: Twenty-four specimens of nonpathological human adrenals from 7 months to 62 years retrieved from autopsy files. Immunohistochemistry for P450 side-chain cleavage (P450scc), 17α hydroxylase (P450c17), dehydroepiandrosterone sulfotransferase (DHEA-ST), P450 oxidoreductase, cytochrome b5, and 3β-hydroxysteroid dehydrogenase (3βHSD) was performed in these specimens, and the immunointensity was evaluated using CAS 200 computed image analysis system. RESULTS: Immunoreactivity of P450scc was marked in the zona glomerulosa, fasciculata and reticularis in the adrenal glands of all the cases examined. P450c17 and DHEA-ST immunoreactivity was weak in the zona fasciculata and reticularis in the adrenals of age 7 months to 5 years, but thereafter became prominent in the zona reticularis. Immunoreactivity of P450 oxidoreductase and cytochrome b5, components of the electron transfer system hypothesized to regulate the 17-20 lyase activity of P450c17, was weak in all three zones of adrenal cortex from 7 months to 5 years, and became more marked in the zona reticularis after age 5 years. 3βHSD immunoreactivity was marked in all three zones of the adrenal cortex from 7 months to 8 years but thereafter decreased in the zona reticularis. CONCLUSIONS: These data suggest that the human adrenal zona reticularis markedly begins to develop morphologically and functionally at around 5 years of age. The increased level of P450c17, DHEA-ST, P450 oxidoreductase, and cytochrome b5, and the decreased level of 3βHSD in the reticularis is likely to contribute to increased C19 steroid production during adrenarche.",
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T1 - Developmental changes in steroidogenic enzymes in human postnatal adrenal cortex

T2 - Immunohistochemical studies

AU - Suzuki, Takashi

AU - Sasano, Hironobu

AU - Takeyama, Junji

AU - Kaneko, Chika

AU - Freije, William A.

AU - Carr, Bruce R.

AU - Rainey, William E.

PY - 2000

Y1 - 2000

N2 - OBJECTIVE: Adrenarche is considered to occur as a result of intra-adrenal changes in steroidogenic enzymes involved in C19 steroid production. The present study was conducted because developmental changes in steroidogenic enzymes have not been examined well in human postnatal adrenal. PATIENTS AND MEASUREMENTS: Twenty-four specimens of nonpathological human adrenals from 7 months to 62 years retrieved from autopsy files. Immunohistochemistry for P450 side-chain cleavage (P450scc), 17α hydroxylase (P450c17), dehydroepiandrosterone sulfotransferase (DHEA-ST), P450 oxidoreductase, cytochrome b5, and 3β-hydroxysteroid dehydrogenase (3βHSD) was performed in these specimens, and the immunointensity was evaluated using CAS 200 computed image analysis system. RESULTS: Immunoreactivity of P450scc was marked in the zona glomerulosa, fasciculata and reticularis in the adrenal glands of all the cases examined. P450c17 and DHEA-ST immunoreactivity was weak in the zona fasciculata and reticularis in the adrenals of age 7 months to 5 years, but thereafter became prominent in the zona reticularis. Immunoreactivity of P450 oxidoreductase and cytochrome b5, components of the electron transfer system hypothesized to regulate the 17-20 lyase activity of P450c17, was weak in all three zones of adrenal cortex from 7 months to 5 years, and became more marked in the zona reticularis after age 5 years. 3βHSD immunoreactivity was marked in all three zones of the adrenal cortex from 7 months to 8 years but thereafter decreased in the zona reticularis. CONCLUSIONS: These data suggest that the human adrenal zona reticularis markedly begins to develop morphologically and functionally at around 5 years of age. The increased level of P450c17, DHEA-ST, P450 oxidoreductase, and cytochrome b5, and the decreased level of 3βHSD in the reticularis is likely to contribute to increased C19 steroid production during adrenarche.

AB - OBJECTIVE: Adrenarche is considered to occur as a result of intra-adrenal changes in steroidogenic enzymes involved in C19 steroid production. The present study was conducted because developmental changes in steroidogenic enzymes have not been examined well in human postnatal adrenal. PATIENTS AND MEASUREMENTS: Twenty-four specimens of nonpathological human adrenals from 7 months to 62 years retrieved from autopsy files. Immunohistochemistry for P450 side-chain cleavage (P450scc), 17α hydroxylase (P450c17), dehydroepiandrosterone sulfotransferase (DHEA-ST), P450 oxidoreductase, cytochrome b5, and 3β-hydroxysteroid dehydrogenase (3βHSD) was performed in these specimens, and the immunointensity was evaluated using CAS 200 computed image analysis system. RESULTS: Immunoreactivity of P450scc was marked in the zona glomerulosa, fasciculata and reticularis in the adrenal glands of all the cases examined. P450c17 and DHEA-ST immunoreactivity was weak in the zona fasciculata and reticularis in the adrenals of age 7 months to 5 years, but thereafter became prominent in the zona reticularis. Immunoreactivity of P450 oxidoreductase and cytochrome b5, components of the electron transfer system hypothesized to regulate the 17-20 lyase activity of P450c17, was weak in all three zones of adrenal cortex from 7 months to 5 years, and became more marked in the zona reticularis after age 5 years. 3βHSD immunoreactivity was marked in all three zones of the adrenal cortex from 7 months to 8 years but thereafter decreased in the zona reticularis. CONCLUSIONS: These data suggest that the human adrenal zona reticularis markedly begins to develop morphologically and functionally at around 5 years of age. The increased level of P450c17, DHEA-ST, P450 oxidoreductase, and cytochrome b5, and the decreased level of 3βHSD in the reticularis is likely to contribute to increased C19 steroid production during adrenarche.

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