Developmental competence of porcine genome-edited zygotes

Maria A. Gil, Cristina A. Martinez, Alicia Nohalez, Inmaculada Parrilla, Jordi Roca, Jun Wu, Pablo J. Ross, Cristina Cuello, Juan C. Izpisua, Emilio A. Martinez

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations

Abstract

Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases—including ZFNs, TALENs, and the CRISPR/Cas9 system—has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.

Original languageEnglish (US)
Pages (from-to)814-821
Number of pages8
JournalMolecular Reproduction and Development
Volume84
Issue number9
DOIs
StatePublished - Sep 2017
Externally publishedYes

Keywords

  • CRISPR/Cas9
  • TALEN
  • ZFN
  • cytoplasmic microinjection
  • in vitro-produced zygotes
  • in vivo-derived zygotes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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