@article{5b32e8d115504899829a1b60025ed4fe,
title = "Developmental competence of porcine genome-edited zygotes",
abstract = "Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases—including ZFNs, TALENs, and the CRISPR/Cas9 system—has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.",
keywords = "CRISPR/Cas9, TALEN, ZFN, cytoplasmic microinjection, in vitro-produced zygotes, in vivo-derived zygotes",
author = "Gil, {Maria A.} and Martinez, {Cristina A.} and Alicia Nohalez and Inmaculada Parrilla and Jordi Roca and Jun Wu and Ross, {Pablo J.} and Cristina Cuello and Izpisua, {Juan C.} and Martinez, {Emilio A.}",
note = "Funding Information: The authors are grateful to Xiomara Lucas and Moises Gonzalvez, for their assistance throughout this work. We thank the staff of the Agropor S.A. and Porcisan S.A. piggeries (Murcia, Spain) for their help and excellent management of animals in some of the studies reported herein. Some of the studies presented in this review were supported in part by the Fundaci{\'o}n S{\'e}neca (GERM 19892/GERM/15), Murcia, Spain. The grant-based to C.A.M. and A.N. are supported by the MINECO (BES-2013-064087 and BES-2013-064069). Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",
year = "2017",
month = sep,
doi = "10.1002/mrd.22829",
language = "English (US)",
volume = "84",
pages = "814--821",
journal = "Molecular Reproduction and Development",
issn = "1040-452X",
publisher = "Wiley-Liss Inc.",
number = "9",
}