Developmental coupling of expression of the ighlinked minor antigen H-40 to membrane immunoglobulin expression

Kathy L. Schreiber, Carol Webb, Philip Tucker, Roy Riblet, James Forman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

C.B-20 (Ighb) but not BALB/c (Igha) mice are able to reject BCL1, a spontaneous B cell leukemia of BALB/c origin. The protective immune response is directed to1 ward the minor histocompatibility (H) antigen, H-40, which can be detected by cytotoxic T lymphocytes and is expressed on surface (s) immunoglobulin positive tumor cells including BCL1 as well as slg+ lymphoblasts activated by lipopolysaccharide. The fact that only slg+ tumor cells and lymphoblasts express H-40 suggests that slg itself may be a component involved in CTL recognition of this antigen. However, this possibility was ruled out by demonstrating that removal of slg from target cells did not prevent H-40-specific CTL recognition. To determine whether H-40 expression was coordinately regulated with that of membrane Ig, we determined whether H-40- slg1 cells acquire H-40 when induced to express slg. We also transfected a functional μ c and v region gene into a slg- lymphoma so that it acquired a slg+ phenotype. In neither case did such slg+ cells acquire H-40. Together, these data indicate that H-40 is expressed on B cells representing a particular stage of differentiation that is coincidental with slg. We previously showed that H-40 and Igh loci are linked on the 12th murine chromosome. In this study we further localized H-40 to a point beyond Aat (formerly Pre-1) near Lm-1, a locus previously described to encode minor H-antigens expressed on lymphomyeloid cells. Thus, H-40 and Lm-1 may represent a gene cluster encoding antigens expressed on subsets of lymphoid and myeloid cells.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalTransplantation
Volume48
Issue number2
StatePublished - Jan 1 1989

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Immunoglobulins
Membranes
Minor Histocompatibility Antigens
B-Cell Leukemia
Antigens
B-Cell Antigen Receptors
Cytotoxic T-Lymphocytes
Myeloid Cells
Multigene Family
Lipopolysaccharides
H antigen
Lymphoma
Neoplasms
B-Lymphocytes
Chromosomes
Lymphocytes
Phenotype
Genes

ASJC Scopus subject areas

  • Transplantation

Cite this

Developmental coupling of expression of the ighlinked minor antigen H-40 to membrane immunoglobulin expression. / Schreiber, Kathy L.; Webb, Carol; Tucker, Philip; Riblet, Roy; Forman, James.

In: Transplantation, Vol. 48, No. 2, 01.01.1989, p. 331-338.

Research output: Contribution to journalArticle

Schreiber, Kathy L. ; Webb, Carol ; Tucker, Philip ; Riblet, Roy ; Forman, James. / Developmental coupling of expression of the ighlinked minor antigen H-40 to membrane immunoglobulin expression. In: Transplantation. 1989 ; Vol. 48, No. 2. pp. 331-338.
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abstract = "C.B-20 (Ighb) but not BALB/c (Igha) mice are able to reject BCL1, a spontaneous B cell leukemia of BALB/c origin. The protective immune response is directed to1 ward the minor histocompatibility (H) antigen, H-40, which can be detected by cytotoxic T lymphocytes and is expressed on surface (s) immunoglobulin positive tumor cells including BCL1 as well as slg+ lymphoblasts activated by lipopolysaccharide. The fact that only slg+ tumor cells and lymphoblasts express H-40 suggests that slg itself may be a component involved in CTL recognition of this antigen. However, this possibility was ruled out by demonstrating that removal of slg from target cells did not prevent H-40-specific CTL recognition. To determine whether H-40 expression was coordinately regulated with that of membrane Ig, we determined whether H-40- slg1 cells acquire H-40 when induced to express slg. We also transfected a functional μ c and v region gene into a slg- lymphoma so that it acquired a slg+ phenotype. In neither case did such slg+ cells acquire H-40. Together, these data indicate that H-40 is expressed on B cells representing a particular stage of differentiation that is coincidental with slg. We previously showed that H-40 and Igh loci are linked on the 12th murine chromosome. In this study we further localized H-40 to a point beyond Aat (formerly Pre-1) near Lm-1, a locus previously described to encode minor H-antigens expressed on lymphomyeloid cells. Thus, H-40 and Lm-1 may represent a gene cluster encoding antigens expressed on subsets of lymphoid and myeloid cells.",
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