Developmental regulation of full-length trkC in the rat sciatic nerve

N. Offenhauser, R. Bohm-Matthaei, P. Tsoulfas, L. Parada, M. Meyer

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

In order to gain insight into potential roles of neurotrophins in Schwann cell biology, the expression of neurotrophin receptors of the trk gene family was investigated in rat sciatic nerve development. This analysis revealed differential regulation of truncated and full-length receptors. TrkA was undetectable even when analysed with a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) method. TrkB was present at the mRNA as well as protein level only in its truncated form. Surprisingly, multiple isoforms of trkC, including full-length forms, were detected in early postnatal nerve. Specific antibodies detected truncated and full-length trkC proteins in Western blotting, and RT-PCR revealed the presence of two full-length isoforms, one of them containing the 14 amino acid kinase insert. In situ hybridisation localized the expression of trkC to a subpopulation of Schwann cells. TrkC receptors are expressed already in nerves from day-16 embryos. In contrast to early postnatal stages, full-length trkC receptors are no longer expressed in adult nerves, which, however, maintain expression of truncated trkC transcripts. The presence of trkC kinases in peripheral nerve suggests a role for neurotrophin-3, the only known trkC ligand, in peripheral nerve development.

Original languageEnglish (US)
Pages (from-to)917-925
Number of pages9
JournalEuropean Journal of Neuroscience
Volume7
Issue number5
DOIs
StatePublished - 1995

Fingerprint

trkC Receptor
Sciatic Nerve
Schwann Cells
Reverse Transcriptase Polymerase Chain Reaction
Peripheral Nerves
Protein Isoforms
Phosphotransferases
Neurotrophin 3
Nerve Growth Factor Receptors
Nerve Growth Factors
In Situ Hybridization
Cell Biology
Embryonic Structures
Western Blotting
Ligands
Amino Acids
Messenger RNA
Antibodies
Genes
Proteins

Keywords

  • Neurotrophin
  • Schwann cell
  • Trkb
  • Truncated receptor
  • Tyrcsine kinase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Offenhauser, N., Bohm-Matthaei, R., Tsoulfas, P., Parada, L., & Meyer, M. (1995). Developmental regulation of full-length trkC in the rat sciatic nerve. European Journal of Neuroscience, 7(5), 917-925. https://doi.org/10.1111/j.1460-9568.1995.tb01079.x

Developmental regulation of full-length trkC in the rat sciatic nerve. / Offenhauser, N.; Bohm-Matthaei, R.; Tsoulfas, P.; Parada, L.; Meyer, M.

In: European Journal of Neuroscience, Vol. 7, No. 5, 1995, p. 917-925.

Research output: Contribution to journalArticle

Offenhauser, N, Bohm-Matthaei, R, Tsoulfas, P, Parada, L & Meyer, M 1995, 'Developmental regulation of full-length trkC in the rat sciatic nerve', European Journal of Neuroscience, vol. 7, no. 5, pp. 917-925. https://doi.org/10.1111/j.1460-9568.1995.tb01079.x
Offenhauser, N. ; Bohm-Matthaei, R. ; Tsoulfas, P. ; Parada, L. ; Meyer, M. / Developmental regulation of full-length trkC in the rat sciatic nerve. In: European Journal of Neuroscience. 1995 ; Vol. 7, No. 5. pp. 917-925.
@article{ebe7d9191baa4f5ebf456668a3e04e14,
title = "Developmental regulation of full-length trkC in the rat sciatic nerve",
abstract = "In order to gain insight into potential roles of neurotrophins in Schwann cell biology, the expression of neurotrophin receptors of the trk gene family was investigated in rat sciatic nerve development. This analysis revealed differential regulation of truncated and full-length receptors. TrkA was undetectable even when analysed with a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) method. TrkB was present at the mRNA as well as protein level only in its truncated form. Surprisingly, multiple isoforms of trkC, including full-length forms, were detected in early postnatal nerve. Specific antibodies detected truncated and full-length trkC proteins in Western blotting, and RT-PCR revealed the presence of two full-length isoforms, one of them containing the 14 amino acid kinase insert. In situ hybridisation localized the expression of trkC to a subpopulation of Schwann cells. TrkC receptors are expressed already in nerves from day-16 embryos. In contrast to early postnatal stages, full-length trkC receptors are no longer expressed in adult nerves, which, however, maintain expression of truncated trkC transcripts. The presence of trkC kinases in peripheral nerve suggests a role for neurotrophin-3, the only known trkC ligand, in peripheral nerve development.",
keywords = "Neurotrophin, Schwann cell, Trkb, Truncated receptor, Tyrcsine kinase",
author = "N. Offenhauser and R. Bohm-Matthaei and P. Tsoulfas and L. Parada and M. Meyer",
year = "1995",
doi = "10.1111/j.1460-9568.1995.tb01079.x",
language = "English (US)",
volume = "7",
pages = "917--925",
journal = "European Journal of Neuroscience",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Developmental regulation of full-length trkC in the rat sciatic nerve

AU - Offenhauser, N.

AU - Bohm-Matthaei, R.

AU - Tsoulfas, P.

AU - Parada, L.

AU - Meyer, M.

PY - 1995

Y1 - 1995

N2 - In order to gain insight into potential roles of neurotrophins in Schwann cell biology, the expression of neurotrophin receptors of the trk gene family was investigated in rat sciatic nerve development. This analysis revealed differential regulation of truncated and full-length receptors. TrkA was undetectable even when analysed with a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) method. TrkB was present at the mRNA as well as protein level only in its truncated form. Surprisingly, multiple isoforms of trkC, including full-length forms, were detected in early postnatal nerve. Specific antibodies detected truncated and full-length trkC proteins in Western blotting, and RT-PCR revealed the presence of two full-length isoforms, one of them containing the 14 amino acid kinase insert. In situ hybridisation localized the expression of trkC to a subpopulation of Schwann cells. TrkC receptors are expressed already in nerves from day-16 embryos. In contrast to early postnatal stages, full-length trkC receptors are no longer expressed in adult nerves, which, however, maintain expression of truncated trkC transcripts. The presence of trkC kinases in peripheral nerve suggests a role for neurotrophin-3, the only known trkC ligand, in peripheral nerve development.

AB - In order to gain insight into potential roles of neurotrophins in Schwann cell biology, the expression of neurotrophin receptors of the trk gene family was investigated in rat sciatic nerve development. This analysis revealed differential regulation of truncated and full-length receptors. TrkA was undetectable even when analysed with a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) method. TrkB was present at the mRNA as well as protein level only in its truncated form. Surprisingly, multiple isoforms of trkC, including full-length forms, were detected in early postnatal nerve. Specific antibodies detected truncated and full-length trkC proteins in Western blotting, and RT-PCR revealed the presence of two full-length isoforms, one of them containing the 14 amino acid kinase insert. In situ hybridisation localized the expression of trkC to a subpopulation of Schwann cells. TrkC receptors are expressed already in nerves from day-16 embryos. In contrast to early postnatal stages, full-length trkC receptors are no longer expressed in adult nerves, which, however, maintain expression of truncated trkC transcripts. The presence of trkC kinases in peripheral nerve suggests a role for neurotrophin-3, the only known trkC ligand, in peripheral nerve development.

KW - Neurotrophin

KW - Schwann cell

KW - Trkb

KW - Truncated receptor

KW - Tyrcsine kinase

UR - http://www.scopus.com/inward/record.url?scp=0029046405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029046405&partnerID=8YFLogxK

U2 - 10.1111/j.1460-9568.1995.tb01079.x

DO - 10.1111/j.1460-9568.1995.tb01079.x

M3 - Article

C2 - 7613627

AN - SCOPUS:0029046405

VL - 7

SP - 917

EP - 925

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 5

ER -