Developmentally programmed gene elimination in Euplotes crassus facilitates a switch in the telomerase catalytic subunit

Zemfira Karamysheva, Libin Wang, Timothy Shrode, Janna Bednenko, Leigh Anne Hurley, Dorothy E. Shippen

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The primary function of telomerase is to maintain preexisting telomere tracts. In the ciliate Euplotes crassus, however, telomerase RNP structure and substrate recognition are altered during macronuclear development to facilitate de novo telomere addition. We found that E. crassus harbors three TERT genes encoding the telomerase catalytic subunit that not only vary in their nucleotide and predicted protein sequences, but also in their expression profiles. Expression of EcTERT-1 and -3 correlates with the requirement for telomere maintenance, while that of EcTERT-2 correlates with de novo telomere synthesis. All three genes appear to require ribosomal frameshifting for expression of catalytically active protein. The transcriptionally active form of EcTERT-2 exists only transiently in mated cells and is absent from the vegetative macronucleus. Thus, telomerase expression in Euplotes is controlled by unique regulatory mechanisms that culminate in a developmental switch to a different catalytic subunit with properties suited to de novo telomere addition.

Original languageEnglish (US)
Pages (from-to)565-576
Number of pages12
JournalCell
Volume113
Issue number5
DOIs
StatePublished - May 30 2003

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Karamysheva, Z., Wang, L., Shrode, T., Bednenko, J., Hurley, L. A., & Shippen, D. E. (2003). Developmentally programmed gene elimination in Euplotes crassus facilitates a switch in the telomerase catalytic subunit. Cell, 113(5), 565-576. https://doi.org/10.1016/S0092-8674(03)00363-5