Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms

M. Kuro-o, R. Nagai, H. Tsuchimochi, H. Katoh, Y. Yazak, A. Ohkubo, F. Takaku

Research output: Contribution to journalArticle

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Abstract

Two types of smooth muscle myosin heavy chain (MHC) isoforms, SM1 and SM2, were recently identified to have different carboxyl termini (Nagai, R., Kuro-o, M., Babji, P., and Periasamy, M. (1989) J. Biol. Chem. 264, 9734-9737). SM1 and SM2 are considered to be generated from a single gene through alternative RNA splicing. In this study we investigated expression of vascular MHC isoforms during development in rabbits at the mRNA, protein, and histological levels. In adults, all smooth muscle cells reacted with both anti-SM1 and anti-SM2 antibodies on immunofluorescence, suggesting the coexpression of SM1 and SM2 in a single cell. In fetal and perinatal rabbits, however, only anti-SM1 antibody consistently reacted with smooth muscles. Reactivity with anti-SM2 antibody was negative in the fetal and neonatal blood vessles and gradually increased during 30 days after birth. These developmental changes in SM1 and SM2 expression at the histological level coincided with mRNA expression of each MHC isoform as determined by S1 nuclease mapping, indicating that expression of SM1 and SM2 is controlled at the level of RNA splicing. However, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of myosin from fetal and perinatal aortas revealed the presence of large amount of SM2. Interestingly, fetal SM2 did not cross-react with our anti-SM2 antibody on immunoblotting. We conclude that expression of SM1 and SM2 are differentially regulated during development and that a third type of MHC isoform may exist in embryonic and perinatal vascular smooth muscles.

Original languageEnglish (US)
Pages (from-to)18272-18275
Number of pages4
JournalJournal of Biological Chemistry
Volume264
Issue number31
StatePublished - 1989

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Smooth Muscle Myosins
Myosin Heavy Chains
Vascular Smooth Muscle
Anti-Idiotypic Antibodies
Protein Isoforms
Muscle
Antibodies
Rabbits
RNA Splicing
Messenger RNA
Alternative Splicing
Myosins
Electrophoresis
Fetal Blood
Immunoblotting
Sodium Dodecyl Sulfate
Smooth Muscle Myocytes
Fluorescent Antibody Technique
Smooth Muscle
Blood Vessels

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kuro-o, M., Nagai, R., Tsuchimochi, H., Katoh, H., Yazak, Y., Ohkubo, A., & Takaku, F. (1989). Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms. Journal of Biological Chemistry, 264(31), 18272-18275.

Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms. / Kuro-o, M.; Nagai, R.; Tsuchimochi, H.; Katoh, H.; Yazak, Y.; Ohkubo, A.; Takaku, F.

In: Journal of Biological Chemistry, Vol. 264, No. 31, 1989, p. 18272-18275.

Research output: Contribution to journalArticle

Kuro-o, M, Nagai, R, Tsuchimochi, H, Katoh, H, Yazak, Y, Ohkubo, A & Takaku, F 1989, 'Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms', Journal of Biological Chemistry, vol. 264, no. 31, pp. 18272-18275.
Kuro-o M, Nagai R, Tsuchimochi H, Katoh H, Yazak Y, Ohkubo A et al. Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms. Journal of Biological Chemistry. 1989;264(31):18272-18275.
Kuro-o, M. ; Nagai, R. ; Tsuchimochi, H. ; Katoh, H. ; Yazak, Y. ; Ohkubo, A. ; Takaku, F. / Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 31. pp. 18272-18275.
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