Diabetes enhances lectin-like oxidized LDL receptor-1 (LOX-1) expression in the vascular endothelium: Possible role of LOX-1 ligand and AGE

Mingyi Chen, Miki Nagase, Toshiro Fujita, Shuh Narumiya, Tomoh Masaki, Tatsuya Sawamura

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Diabetes mellitus accelerating atherosclerosis was associated with the enhanced glycoxidative modification of lipoproteins. LOX-1, the endothelial oxidized LDL receptor might be involved in the pathogenesis of diabetic atherosclerosis. In this study, we examined the vascular expression of LOX-1 in streptozotocin-induced diabetic rats. We found that LOX-1 was significantly increased in diabetic rat aorta compared with nondiabetic control. Immunohistochemistry revealed that the most distinctive staining of LOX-1 was in the endothelial cells, especially in the bifurcations of artery branches from aorta. In cultured aortic endothelial cells, diabetic rat serum and advanced glycation endproducts-BSA induced LOX-1 expression, while control rat serum along with high glucose did not. Applying a competitive inhibition assay, we found that LOX-1 ligand activity was accumulated in the diabetic rat serum, mainly in VLDL/LDL fractions. In addition, VLDL/LDL prominently increased LOX-1 among all the lipoprotein fractions of diabetic rat serum. In conclusion, diabetes markedly upregulated LOX-1 expression in the aortic endothelial cells. The enhanced glycoxidative modification of lipoproteins may contribute to the underlying mechanisms.

Original languageEnglish (US)
Pages (from-to)962-968
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume287
Issue number4
DOIs
StatePublished - Oct 5 2001

Fingerprint

Oxidized LDL Receptors
Vascular Endothelium
Medical problems
LDL Lipoproteins
Lectins
Ligands
Rats
Endothelial cells
Lipoproteins
Endothelial Cells
Serum
Aorta
Atherosclerosis
Rat control
Streptozocin
Blood Vessels
Assays
Diabetes Mellitus
Arteries
Immunohistochemistry

Keywords

  • Advanced glycation endproducts
  • Atherosclerosis
  • Diabetes mellitus
  • Endothelium
  • LOX-1
  • OxLDL

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Diabetes enhances lectin-like oxidized LDL receptor-1 (LOX-1) expression in the vascular endothelium : Possible role of LOX-1 ligand and AGE. / Chen, Mingyi; Nagase, Miki; Fujita, Toshiro; Narumiya, Shuh; Masaki, Tomoh; Sawamura, Tatsuya.

In: Biochemical and Biophysical Research Communications, Vol. 287, No. 4, 05.10.2001, p. 962-968.

Research output: Contribution to journalArticle

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