Diagnosis of encephalitozoonosis in experimentally infected rabbits by intradermal and immunofluorescence tests

N. J. Wosu, J. A. Shadduck, S. P. Pakes, J. K. Frenkel, K. S. Todd, J. D. Conroy

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

The indirect immunofluorescence antibody test was performed on serial blood samples from eight young New Zealand White rabbits with experimental encephalitozoonosis. The test showed seroconversion in six of the eight infected rabbits by the 8th day after inoculation and in all rabbits by the 15th day. Antibody titers reached a peak by about the 36th day after inoculation and remained significantly elevated until the termination of the experiment at 84 days after inoculation. None of four sham-inoculated rabbits showed an immunofluorescence response by the 60th day after inoculation. Immunofluorescence and intradermal test responses were compared before infection and at the 60th day after inoculation in a total of 32 experimentally infected rabbits. Both tests were equally effective (100%) in detecting infected animals. Six of eight (first group) and 22 of 24 (second group) experimentally infected rabbits were confirmed histologically to have lesions compatible with encephalitozoonosis. No cross reactions were observed between Encephalitozoon cuniculi and Toxoplasma gondii, Eimeria perforans, or Eimeria stiedai by intradermal test or immunofluorescence test.

Original languageEnglish (US)
Title of host publicationLaboratory Animal Science
Pages210-216
Number of pages7
Volume27
Edition2
StatePublished - 1977

ASJC Scopus subject areas

  • Medicine(all)

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