Diagnostic markers in paediatric medulloblastoma: A paediatric oncology group study

R. E. McLendon, H. S. Friedman, H. E. Fuchs, L. E. Kun, S. H. Bigner

Research output: Contribution to journalArticle

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Abstract

Aims: We have reviewed immunohistochemically 17 paediatric medulloblastomas in order to determine if correlations exist that might be useful in subclassifying these tumours. Methods and results: The patient group included 11 children who had died (mean survival 13 months) and six still alive (followed for up to 10 years). Ten tumours were diffuse and six were nodular (one biopsy had only perivascular tumour). Of the 10 diffuse tumours, three were desmoplastic; of the six nodular tumours, all six were desmoplastic. All 17 tumours were synaptophysin-reactive; three nodular tumours were glial fibrillary acidic protein (GFAP)-reactive in the nodules (two of three S100-reactive tumours were also GFAP-reactive). MIB-1 labelling indices (LI) ranged from 5 to 80%. Six tumours exhibited at least 1% LI against Tp53 (Mab D07 and/or Mab 1801). Eight cases were 100% bcl2 reactive with nine cases having an LI<80% ('low labelling'). All nine 'low labelling' bcl2 cases were TP53 non-reactive; all six Tp53-reactive cases were bcl2 100% reactive. Six of 10 patients with diffuse medulloblastomas survived 18 months or less while four of 10 are alive up to 10 years. In contrast, five of six patients with nodular neoplasms died within 48 months of diagnosis with one patient followed up for less than 1 year. Conclusions: Immunohistochemistry is a useful adjunct in characterizing subsets of paediatric medulloblastomas and confirms that larger co-operative studies may be fruitful in identifying a prognostic utility of a combined histochemical/immunohistochemical analysis on these tumours.

Original languageEnglish (US)
Pages (from-to)154-162
Number of pages9
JournalHistopathology
Volume34
Issue number2
DOIs
StatePublished - Feb 23 1999

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Medulloblastoma
Pediatrics
Neoplasms
Glial Fibrillary Acidic Protein
Synaptophysin
Immunohistochemistry

Keywords

  • Immunohistochemistry
  • Medulloblastoma
  • Paediatric
  • Pathology

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

Diagnostic markers in paediatric medulloblastoma : A paediatric oncology group study. / McLendon, R. E.; Friedman, H. S.; Fuchs, H. E.; Kun, L. E.; Bigner, S. H.

In: Histopathology, Vol. 34, No. 2, 23.02.1999, p. 154-162.

Research output: Contribution to journalArticle

McLendon, R. E. ; Friedman, H. S. ; Fuchs, H. E. ; Kun, L. E. ; Bigner, S. H. / Diagnostic markers in paediatric medulloblastoma : A paediatric oncology group study. In: Histopathology. 1999 ; Vol. 34, No. 2. pp. 154-162.
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abstract = "Aims: We have reviewed immunohistochemically 17 paediatric medulloblastomas in order to determine if correlations exist that might be useful in subclassifying these tumours. Methods and results: The patient group included 11 children who had died (mean survival 13 months) and six still alive (followed for up to 10 years). Ten tumours were diffuse and six were nodular (one biopsy had only perivascular tumour). Of the 10 diffuse tumours, three were desmoplastic; of the six nodular tumours, all six were desmoplastic. All 17 tumours were synaptophysin-reactive; three nodular tumours were glial fibrillary acidic protein (GFAP)-reactive in the nodules (two of three S100-reactive tumours were also GFAP-reactive). MIB-1 labelling indices (LI) ranged from 5 to 80{\%}. Six tumours exhibited at least 1{\%} LI against Tp53 (Mab D07 and/or Mab 1801). Eight cases were 100{\%} bcl2 reactive with nine cases having an LI<80{\%} ('low labelling'). All nine 'low labelling' bcl2 cases were TP53 non-reactive; all six Tp53-reactive cases were bcl2 100{\%} reactive. Six of 10 patients with diffuse medulloblastomas survived 18 months or less while four of 10 are alive up to 10 years. In contrast, five of six patients with nodular neoplasms died within 48 months of diagnosis with one patient followed up for less than 1 year. Conclusions: Immunohistochemistry is a useful adjunct in characterizing subsets of paediatric medulloblastomas and confirms that larger co-operative studies may be fruitful in identifying a prognostic utility of a combined histochemical/immunohistochemical analysis on these tumours.",
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