Diagnostic utility and clinical significance of naso- and oropharyngeal samples used in a PCR assay to diagnose Mycoplasma pneumoniae infection in children with community-acquired pneumonia

Ian C. Michelow, Kurt Olsen, Juanita Lozano, Lynn B. Duffy, George H. McCracken, R. Doug Hardy

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

PCR assays of naso- and oropharyngeal samples among hospitalized children appear equally effective for the diagnosis of serologically confirmed community-acquired mycoplasmal pneumonia. However, the combination of results from both sites yields optimal sensitivity (57%), specificity (98%), and positive (92%) and negative (82%) predictive values when compared with Mycoplasma pneumoniae enzyme-linked immunosorbent assay.

Original languageEnglish (US)
Pages (from-to)3339-3341
Number of pages3
JournalJournal of Clinical Microbiology
Volume42
Issue number7
DOIs
StatePublished - Jul 2004

Fingerprint

Mycoplasma pneumoniae
Hospitalized Child
Pneumonia
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
Polymerase Chain Reaction
Infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Diagnostic utility and clinical significance of naso- and oropharyngeal samples used in a PCR assay to diagnose Mycoplasma pneumoniae infection in children with community-acquired pneumonia. / Michelow, Ian C.; Olsen, Kurt; Lozano, Juanita; Duffy, Lynn B.; McCracken, George H.; Hardy, R. Doug.

In: Journal of Clinical Microbiology, Vol. 42, No. 7, 07.2004, p. 3339-3341.

Research output: Contribution to journalArticle

@article{c19eef362d1a43f58e642972145f6528,
title = "Diagnostic utility and clinical significance of naso- and oropharyngeal samples used in a PCR assay to diagnose Mycoplasma pneumoniae infection in children with community-acquired pneumonia",
abstract = "PCR assays of naso- and oropharyngeal samples among hospitalized children appear equally effective for the diagnosis of serologically confirmed community-acquired mycoplasmal pneumonia. However, the combination of results from both sites yields optimal sensitivity (57{\%}), specificity (98{\%}), and positive (92{\%}) and negative (82{\%}) predictive values when compared with Mycoplasma pneumoniae enzyme-linked immunosorbent assay.",
author = "Michelow, {Ian C.} and Kurt Olsen and Juanita Lozano and Duffy, {Lynn B.} and McCracken, {George H.} and Hardy, {R. Doug}",
year = "2004",
month = "7",
doi = "10.1128/JCM.42.7.3339-3341.2004",
language = "English (US)",
volume = "42",
pages = "3339--3341",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "7",

}

TY - JOUR

T1 - Diagnostic utility and clinical significance of naso- and oropharyngeal samples used in a PCR assay to diagnose Mycoplasma pneumoniae infection in children with community-acquired pneumonia

AU - Michelow, Ian C.

AU - Olsen, Kurt

AU - Lozano, Juanita

AU - Duffy, Lynn B.

AU - McCracken, George H.

AU - Hardy, R. Doug

PY - 2004/7

Y1 - 2004/7

N2 - PCR assays of naso- and oropharyngeal samples among hospitalized children appear equally effective for the diagnosis of serologically confirmed community-acquired mycoplasmal pneumonia. However, the combination of results from both sites yields optimal sensitivity (57%), specificity (98%), and positive (92%) and negative (82%) predictive values when compared with Mycoplasma pneumoniae enzyme-linked immunosorbent assay.

AB - PCR assays of naso- and oropharyngeal samples among hospitalized children appear equally effective for the diagnosis of serologically confirmed community-acquired mycoplasmal pneumonia. However, the combination of results from both sites yields optimal sensitivity (57%), specificity (98%), and positive (92%) and negative (82%) predictive values when compared with Mycoplasma pneumoniae enzyme-linked immunosorbent assay.

UR - http://www.scopus.com/inward/record.url?scp=3142776277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142776277&partnerID=8YFLogxK

U2 - 10.1128/JCM.42.7.3339-3341.2004

DO - 10.1128/JCM.42.7.3339-3341.2004

M3 - Article

C2 - 15243111

AN - SCOPUS:3142776277

VL - 42

SP - 3339

EP - 3341

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 7

ER -