Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila

Feng Jiang; Xuecheng Ye; Xiang Liu; Lauren Fincher; Dennis McKearin; Qinghua Liu

doi:10.1101/gad.1334005

Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila

Feng Jiang, Xuecheng Ye, Xiang Liu, Lauren Fincher, Dennis McKearin, Qinghua Liu

Research output: Contribution to journal › Article › peer-review

246 Scopus citations

Abstract

In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNAbinding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.

Original language	English (US)
Pages (from-to)	1674-1679
Number of pages	6
Journal	Genes and Development
Volume	19
Issue number	14
DOIs	https://doi.org/10.1101/gad.1334005
State	Published - Jul 2005

Keywords

Dicer-1
Pre-miRNA
R3D1-L
RNase III
dsRNA binding
miRNA

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1101/gad.1334005

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title = "Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila",

abstract = "In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNAbinding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.",

keywords = "Dicer-1, Pre-miRNA, R3D1-L, RNase III, dsRNA binding, miRNA",

author = "Feng Jiang and Xuecheng Ye and Xiang Liu and Lauren Fincher and Dennis McKearin and Qinghua Liu",

year = "2005",

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doi = "10.1101/gad.1334005",

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T1 - Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila

AU - Jiang, Feng

AU - Ye, Xuecheng

AU - Liu, Xiang

AU - Fincher, Lauren

AU - McKearin, Dennis

AU - Liu, Qinghua

PY - 2005/7

Y1 - 2005/7

N2 - In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNAbinding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.

AB - In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNAbinding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.

KW - Dicer-1

KW - Pre-miRNA

KW - R3D1-L

KW - RNase III

KW - dsRNA binding

KW - miRNA

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Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila

Abstract

Keywords

ASJC Scopus subject areas

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