Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H nuclear magnetic resonance search for candidate biomarkers

Adam Feliks Junka, Stanisław Deja, Danuta Smutnicka, Patrycja Szymczyk, Grzegorz Ziółkowski, Marzenna Bartoszewicz, Piotr Młynarz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.

Original languageEnglish (US)
Pages (from-to)701-706
Number of pages6
JournalActa Biochimica Polonica
Volume60
Issue number4
StatePublished - Dec 1 2013
Externally publishedYes

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Metabolome
Biofilms
Biomarkers
Staphylococcus aureus
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Infection
X-Ray Microtomography
Betaine
Isoleucine
Metabolites
Principal Component Analysis
Alanine
Electron Scanning Microscopy
Principal component analysis
Nuclear magnetic resonance spectroscopy
X rays
Scanning electron microscopy

Keywords

  • Biofilm
  • Glycine-betaine
  • Metabolomics
  • NMR
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H nuclear magnetic resonance search for candidate biomarkers. / Junka, Adam Feliks; Deja, Stanisław; Smutnicka, Danuta; Szymczyk, Patrycja; Ziółkowski, Grzegorz; Bartoszewicz, Marzenna; Młynarz, Piotr.

In: Acta Biochimica Polonica, Vol. 60, No. 4, 01.12.2013, p. 701-706.

Research output: Contribution to journalArticle

Junka, AF, Deja, S, Smutnicka, D, Szymczyk, P, Ziółkowski, G, Bartoszewicz, M & Młynarz, P 2013, 'Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H nuclear magnetic resonance search for candidate biomarkers', Acta Biochimica Polonica, vol. 60, no. 4, pp. 701-706.
Junka, Adam Feliks ; Deja, Stanisław ; Smutnicka, Danuta ; Szymczyk, Patrycja ; Ziółkowski, Grzegorz ; Bartoszewicz, Marzenna ; Młynarz, Piotr. / Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H nuclear magnetic resonance search for candidate biomarkers. In: Acta Biochimica Polonica. 2013 ; Vol. 60, No. 4. pp. 701-706.
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