TY - JOUR
T1 - Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H nuclear magnetic resonance search for candidate biomarkers
AU - Junka, Adam Feliks
AU - Deja, Stanisław
AU - Smutnicka, Danuta
AU - Szymczyk, Patrycja
AU - Ziółkowski, Grzegorz
AU - Bartoszewicz, Marzenna
AU - Młynarz, Piotr
PY - 2013
Y1 - 2013
N2 - Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.
AB - Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.
KW - Biofilm
KW - Glycine-betaine
KW - Metabolomics
KW - NMR
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=84893416472&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893416472&partnerID=8YFLogxK
M3 - Article
C2 - 24432320
AN - SCOPUS:84893416472
SN - 0001-527X
VL - 60
SP - 701
EP - 706
JO - Acta Biochimica Polonica
JF - Acta Biochimica Polonica
IS - 4
ER -