Differences in response of myosin isozyme transition of ordinary and specialized myocardium to overload

K. Nomoto, I. Komuro, M. Kuro-o, H. Tsuchimochi, F. Takaku, K. Machii, Y. Yazaki

Research output: Contribution to journalArticle

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Abstract

To investigate the response of myosin isozyme transition in specialized myocardium to cardiac overload, we examined immunohistochemically the distribution of myosin isozymes in sinus node cells of overloaded canine atria, using the monoclonal antibodies CMA19 and HMC14, which are specific for atrial myosin heavy chain (α-HC) and ventricular myosin heavy chain (β-HC), respectively. Overloading in canine right atria was induced by artificial tricuspid valve regurgitation and pulmonary stenosis. Right atrial mena pressure rose to 15-20 mm Hg (n=4) 2 months after surgery. In the working myocardium, cardiac overload caused redistribution of myosin isozymes, α-HC to β-HC. Compared with the normal right atria, fewer myocytes were labeled with CMA19, but more were labeled with HMC14. However, the reactivity of sinus node cells with CMA19 and HMC14 was not changed between normal and overloaded right atria, indicating no redistribution of myosin heavy chain isozymes, α-HC to β-HC. These results suggest that isozymes in myosin heavy chains in the specialized myocardium are protected from overload effects by their firm cytoskeletal framework or other mechanisms.

Original languageEnglish (US)
Pages (from-to)1088-1092
Number of pages5
JournalCirculation Research
Volume62
Issue number6
StatePublished - 1988

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Myosins
Myosin Heavy Chains
Isoenzymes
Myocardium
Heart Atria
Sinoatrial Node
Atrial Myosins
Tricuspid Valve Stenosis
Canidae
Ventricular Myosins
Tricuspid Valve Insufficiency
Pulmonary Valve Stenosis
Atrial Pressure
Muscle Cells
Monoclonal Antibodies

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Nomoto, K., Komuro, I., Kuro-o, M., Tsuchimochi, H., Takaku, F., Machii, K., & Yazaki, Y. (1988). Differences in response of myosin isozyme transition of ordinary and specialized myocardium to overload. Circulation Research, 62(6), 1088-1092.

Differences in response of myosin isozyme transition of ordinary and specialized myocardium to overload. / Nomoto, K.; Komuro, I.; Kuro-o, M.; Tsuchimochi, H.; Takaku, F.; Machii, K.; Yazaki, Y.

In: Circulation Research, Vol. 62, No. 6, 1988, p. 1088-1092.

Research output: Contribution to journalArticle

Nomoto, K, Komuro, I, Kuro-o, M, Tsuchimochi, H, Takaku, F, Machii, K & Yazaki, Y 1988, 'Differences in response of myosin isozyme transition of ordinary and specialized myocardium to overload', Circulation Research, vol. 62, no. 6, pp. 1088-1092.
Nomoto K, Komuro I, Kuro-o M, Tsuchimochi H, Takaku F, Machii K et al. Differences in response of myosin isozyme transition of ordinary and specialized myocardium to overload. Circulation Research. 1988;62(6):1088-1092.
Nomoto, K. ; Komuro, I. ; Kuro-o, M. ; Tsuchimochi, H. ; Takaku, F. ; Machii, K. ; Yazaki, Y. / Differences in response of myosin isozyme transition of ordinary and specialized myocardium to overload. In: Circulation Research. 1988 ; Vol. 62, No. 6. pp. 1088-1092.
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