Differences in the critical period for regenerative growth of supraspinal axons across the lesion site after transection of the thoracic cord in developing opossums

G. F. Martin, J. R. Terman, X. M. Wang

Research output: Contribution to journalArticle

Abstract

When the spinal cord is transected in adult opossums, regeneration of cut axons does not occur. Regeneration of descending axons has been documented, however, when the lesion is made early in development. In the present study, we asked whether the critical period for regenerative growth is the same for all axons. Opossums are useful for such studies because they are born in a very immature state and, after appropriate anesthesia, their spinal cord can be manipulated in a feta-like stage of development without intrauterine surgery. Supraspinal neurons that innervate the lumbar cord on PD5, 15 and 20 were prelabeled by lumbar injectons of Fast Blue (FB). Five days later, FB was removed and the spinal cord was transected at thoracic levels. Fourteen days later, Rhodamine B Dextran (RBD) was injected between the site of the* FB injection and the lesion. The pups were maintained for an additional 7-10 days before sacrifice and perfusion. We assumed that FB labeled neurons in the brain survived axotomy and that those which also contained RBD supported regenerated axons. In the PD5 group (lesioned at PD10), regenerative growth could be documented for axons originating in all of the supraspinal nuclei that innervate the lumbar cord by PD10. When the injections and lesions were made at the later ages, however, neurons which supported regenerative growth were fewer in number and regionally restricted. In some cases, they were limited primarily to the red nucleus and raphe. Our results support our previous contention that regeneration of cut axons contributes to growth across the lesion after transection of the thoracic cord in developing opossums, but they also show that the critical period for regeneration is not the same for all axons.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

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Opossums
opossums
chest
axons
lesions (animal)
Axons
Spinal Cord
rhodamine B
Growth
Regeneration
spinal cord
Neurons
neurons
dextran
Dextrans
injection
Red Nucleus
Axotomy
Injections
pups

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

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title = "Differences in the critical period for regenerative growth of supraspinal axons across the lesion site after transection of the thoracic cord in developing opossums",
abstract = "When the spinal cord is transected in adult opossums, regeneration of cut axons does not occur. Regeneration of descending axons has been documented, however, when the lesion is made early in development. In the present study, we asked whether the critical period for regenerative growth is the same for all axons. Opossums are useful for such studies because they are born in a very immature state and, after appropriate anesthesia, their spinal cord can be manipulated in a feta-like stage of development without intrauterine surgery. Supraspinal neurons that innervate the lumbar cord on PD5, 15 and 20 were prelabeled by lumbar injectons of Fast Blue (FB). Five days later, FB was removed and the spinal cord was transected at thoracic levels. Fourteen days later, Rhodamine B Dextran (RBD) was injected between the site of the* FB injection and the lesion. The pups were maintained for an additional 7-10 days before sacrifice and perfusion. We assumed that FB labeled neurons in the brain survived axotomy and that those which also contained RBD supported regenerated axons. In the PD5 group (lesioned at PD10), regenerative growth could be documented for axons originating in all of the supraspinal nuclei that innervate the lumbar cord by PD10. When the injections and lesions were made at the later ages, however, neurons which supported regenerative growth were fewer in number and regionally restricted. In some cases, they were limited primarily to the red nucleus and raphe. Our results support our previous contention that regeneration of cut axons contributes to growth across the lesion after transection of the thoracic cord in developing opossums, but they also show that the critical period for regeneration is not the same for all axons.",
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AU - Terman, J. R.

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N2 - When the spinal cord is transected in adult opossums, regeneration of cut axons does not occur. Regeneration of descending axons has been documented, however, when the lesion is made early in development. In the present study, we asked whether the critical period for regenerative growth is the same for all axons. Opossums are useful for such studies because they are born in a very immature state and, after appropriate anesthesia, their spinal cord can be manipulated in a feta-like stage of development without intrauterine surgery. Supraspinal neurons that innervate the lumbar cord on PD5, 15 and 20 were prelabeled by lumbar injectons of Fast Blue (FB). Five days later, FB was removed and the spinal cord was transected at thoracic levels. Fourteen days later, Rhodamine B Dextran (RBD) was injected between the site of the* FB injection and the lesion. The pups were maintained for an additional 7-10 days before sacrifice and perfusion. We assumed that FB labeled neurons in the brain survived axotomy and that those which also contained RBD supported regenerated axons. In the PD5 group (lesioned at PD10), regenerative growth could be documented for axons originating in all of the supraspinal nuclei that innervate the lumbar cord by PD10. When the injections and lesions were made at the later ages, however, neurons which supported regenerative growth were fewer in number and regionally restricted. In some cases, they were limited primarily to the red nucleus and raphe. Our results support our previous contention that regeneration of cut axons contributes to growth across the lesion after transection of the thoracic cord in developing opossums, but they also show that the critical period for regeneration is not the same for all axons.

AB - When the spinal cord is transected in adult opossums, regeneration of cut axons does not occur. Regeneration of descending axons has been documented, however, when the lesion is made early in development. In the present study, we asked whether the critical period for regenerative growth is the same for all axons. Opossums are useful for such studies because they are born in a very immature state and, after appropriate anesthesia, their spinal cord can be manipulated in a feta-like stage of development without intrauterine surgery. Supraspinal neurons that innervate the lumbar cord on PD5, 15 and 20 were prelabeled by lumbar injectons of Fast Blue (FB). Five days later, FB was removed and the spinal cord was transected at thoracic levels. Fourteen days later, Rhodamine B Dextran (RBD) was injected between the site of the* FB injection and the lesion. The pups were maintained for an additional 7-10 days before sacrifice and perfusion. We assumed that FB labeled neurons in the brain survived axotomy and that those which also contained RBD supported regenerated axons. In the PD5 group (lesioned at PD10), regenerative growth could be documented for axons originating in all of the supraspinal nuclei that innervate the lumbar cord by PD10. When the injections and lesions were made at the later ages, however, neurons which supported regenerative growth were fewer in number and regionally restricted. In some cases, they were limited primarily to the red nucleus and raphe. Our results support our previous contention that regeneration of cut axons contributes to growth across the lesion after transection of the thoracic cord in developing opossums, but they also show that the critical period for regeneration is not the same for all axons.

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