Different biosynthetic transport routes to the plasma membrane in BHK and CHO cells

Tamotsu Yoshimori, Patrick Keller, Michael G. Roth, Kai Simons

Research output: Contribution to journalArticle

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Abstract

The question of how membrane proteins are delivered from the TGN to the cell surface in fibroblasts has received little attention. In this paper we have studied how their post-Golgi delivery routes compare with those in epithelial cells. We have analyzed the transport of the vesicular stomatitis virus G protein, the Semliki Forest virus spike-glycoprotein, both basolateral in MDCK cells, and the influenza virus hemagglutinin, apical in MDCK cells. In addition, we also have studied the transport of a hemagglutinin mutant (Cys543Tyr) which is basolateral in MDCK cells. Aluminum fluoride, a general activator of heterotrimeric G proteins, inhibited the transport of the basolateral cognate proteins, as well as of the hemagglutinin mutant, from the TGN to the cell surface in BHK and CHO cells, while having no effect on the surface delivery of the wild-type hemagglutinin. Only wild-type hemagglutinin became insoluble in the detergent CHAPS during transport through the BHK and CHO Golgi complexes, whereas the basolateral marker proteins remained CHAPS-soluble. We also have developed an in vitro assay using streptolysin O-permeabilized BHK cells, similar to the one we have previously used for analyzing polarized transport in MDCK cells (Pimplikar, S.W., E. Ikonen, and K. Simons. 1994. J. Cell Biol. 125:10251035). In this assay anti-NSF and rab-GDI inhibited transport of Semliki Forest virus spike glycoproteins from the TGN to the cell surface while having little effect on transport of the hemagglutinin. Altogether these data suggest that fibroblasts have apical and basolateral cognate routes from the TGN to the plasma membrane.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalJournal of Cell Biology
Volume133
Issue number2
DOIs
StatePublished - Apr 1996

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CHO Cells
Hemagglutinins
Madin Darby Canine Kidney Cells
Cell Membrane
Semliki forest virus
Glycoproteins
Fibroblasts
Heterotrimeric GTP-Binding Proteins
Golgi Apparatus
Protein Transport
Orthomyxoviridae
Detergents
Membrane Proteins
Proteins
Epithelial Cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Different biosynthetic transport routes to the plasma membrane in BHK and CHO cells. / Yoshimori, Tamotsu; Keller, Patrick; Roth, Michael G.; Simons, Kai.

In: Journal of Cell Biology, Vol. 133, No. 2, 04.1996, p. 247-256.

Research output: Contribution to journalArticle

Yoshimori, Tamotsu ; Keller, Patrick ; Roth, Michael G. ; Simons, Kai. / Different biosynthetic transport routes to the plasma membrane in BHK and CHO cells. In: Journal of Cell Biology. 1996 ; Vol. 133, No. 2. pp. 247-256.
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