Different inhibition characteristics of intracellular transglutaminase activity by cystamine and cysteamine

Ju Hong Jeon, Hye Jin Lee, Gi Yong Jang, Chai Wan Kim, Dong Myung Shin, Sung Yup Cho, Eui Ju Yeo, Sang Chul Park, In Gyu Kim

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The treatment of cystamine, a transglutaminase (TGase) inhibitor, has beneficial effects in several diseases including CAG-expansion disorders and cataract. We compared the inhibition characteristics of cystamine with those of cysteamine, a reduced form of cystamine expected to be present inside cells. Cystamine is a more potent inhibitor for TGase than cysteamine with different kinetics pattern in a non-reducing condition. By contrast, under reducing conditions, the inhibitory effect of cystamine was comparable with that of cysteamine. However, cystamine inhibited intracellular TGase activity more strongly than cysteamine despite of cytoplasmic reducing environment, suggesting that cystamine itself inhibits in situ TGase activity by forming mixed disulfides.

Original languageEnglish (US)
Pages (from-to)576-581
Number of pages6
JournalExperimental and Molecular Medicine
Volume36
Issue number6
DOIs
StatePublished - Dec 31 2004

Keywords

  • Cystamine
  • Cysteamine
  • Inhibitor
  • Polyamine
  • Transglutaminase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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    Jeon, J. H., Lee, H. J., Jang, G. Y., Kim, C. W., Shin, D. M., Cho, S. Y., Yeo, E. J., Park, S. C., & Kim, I. G. (2004). Different inhibition characteristics of intracellular transglutaminase activity by cystamine and cysteamine. Experimental and Molecular Medicine, 36(6), 576-581. https://doi.org/10.1038/emm.2004.74