Differential expression of c-fos in a mouse model of fetal alcohol syndrome

Sarah H. Poggi, Katie M. Goodwin, Joanna M. Hill, Douglas E. Brenneman, Elisabetta Tendi, Sergio Schninelli, Catherine Y. Spong

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

OBJECTIVE: Fetal alcohol syndrome (FAS) results in stillbirth, fetal growth restriction, and mental retardation with injury attributed to oxidative stress. Our objective was to identify signal transduction pathways expressed in a model of FAS and to quantify expression of c-fos, a gene in the stress signal pathway. STUDY DESIGN: Timed, pregnant C57BI6/J mice were injected on E8 with saline solution or alcohol. RNA was extracted from decidua and embryo 6 and 24 hours later. Microarray analysis was used to screen gene pathways. Differential gene expression was confirmed using real-time polymerase chain reaction with results presented as the ratio of c-fos concentration to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). RESULTS: Differential gene expression between alcohol and control was noted for stress signal pathway genes including c-fos. Real-time polymerase chain reaction demonstrated that c-fos messenger RNA expression was greater in the alcohol than control decidua at 6 hours after injection (P < .01). This effect persisted at 24 hours (P < .01). There was no difference in c-fos expression in embryos whose mothers received alcohol versus control after 6 hours (P = .12) or 24 hours (P = .89). CONCLUSION: Alcohol administration during pregnancy results in differential gene expression in the stress signal pathway, particularly in c-fos. C-fos expression in the decidua increases from 6 to 24 hours after alcohol injection, but does not change in the embryo, which may contribute to alcohol-induced damage in FAS.

Original languageEnglish (US)
Pages (from-to)786-789
Number of pages4
JournalAmerican journal of obstetrics and gynecology
Volume189
Issue number3
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

Fetal Alcohol Spectrum Disorders
Alcohols
Decidua
Signal Transduction
fos Genes
Embryonic Structures
Gene Expression
Real-Time Polymerase Chain Reaction
Injections
Glyceraldehyde-3-Phosphate Dehydrogenases
Stillbirth
Fetal Growth Retardation
Microarray Analysis
Sodium Chloride
Intellectual Disability
Oxidative Stress
RNA
Pregnancy
Messenger RNA
Wounds and Injuries

Keywords

  • c-fos
  • Fetal alcohol syndrome
  • Mouse
  • Oxidative stress

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Differential expression of c-fos in a mouse model of fetal alcohol syndrome. / Poggi, Sarah H.; Goodwin, Katie M.; Hill, Joanna M.; Brenneman, Douglas E.; Tendi, Elisabetta; Schninelli, Sergio; Spong, Catherine Y.

In: American journal of obstetrics and gynecology, Vol. 189, No. 3, 01.09.2003, p. 786-789.

Research output: Contribution to journalArticle

Poggi, Sarah H. ; Goodwin, Katie M. ; Hill, Joanna M. ; Brenneman, Douglas E. ; Tendi, Elisabetta ; Schninelli, Sergio ; Spong, Catherine Y. / Differential expression of c-fos in a mouse model of fetal alcohol syndrome. In: American journal of obstetrics and gynecology. 2003 ; Vol. 189, No. 3. pp. 786-789.
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abstract = "OBJECTIVE: Fetal alcohol syndrome (FAS) results in stillbirth, fetal growth restriction, and mental retardation with injury attributed to oxidative stress. Our objective was to identify signal transduction pathways expressed in a model of FAS and to quantify expression of c-fos, a gene in the stress signal pathway. STUDY DESIGN: Timed, pregnant C57BI6/J mice were injected on E8 with saline solution or alcohol. RNA was extracted from decidua and embryo 6 and 24 hours later. Microarray analysis was used to screen gene pathways. Differential gene expression was confirmed using real-time polymerase chain reaction with results presented as the ratio of c-fos concentration to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). RESULTS: Differential gene expression between alcohol and control was noted for stress signal pathway genes including c-fos. Real-time polymerase chain reaction demonstrated that c-fos messenger RNA expression was greater in the alcohol than control decidua at 6 hours after injection (P < .01). This effect persisted at 24 hours (P < .01). There was no difference in c-fos expression in embryos whose mothers received alcohol versus control after 6 hours (P = .12) or 24 hours (P = .89). CONCLUSION: Alcohol administration during pregnancy results in differential gene expression in the stress signal pathway, particularly in c-fos. C-fos expression in the decidua increases from 6 to 24 hours after alcohol injection, but does not change in the embryo, which may contribute to alcohol-induced damage in FAS.",
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AU - Goodwin, Katie M.

AU - Hill, Joanna M.

AU - Brenneman, Douglas E.

AU - Tendi, Elisabetta

AU - Schninelli, Sergio

AU - Spong, Catherine Y.

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N2 - OBJECTIVE: Fetal alcohol syndrome (FAS) results in stillbirth, fetal growth restriction, and mental retardation with injury attributed to oxidative stress. Our objective was to identify signal transduction pathways expressed in a model of FAS and to quantify expression of c-fos, a gene in the stress signal pathway. STUDY DESIGN: Timed, pregnant C57BI6/J mice were injected on E8 with saline solution or alcohol. RNA was extracted from decidua and embryo 6 and 24 hours later. Microarray analysis was used to screen gene pathways. Differential gene expression was confirmed using real-time polymerase chain reaction with results presented as the ratio of c-fos concentration to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). RESULTS: Differential gene expression between alcohol and control was noted for stress signal pathway genes including c-fos. Real-time polymerase chain reaction demonstrated that c-fos messenger RNA expression was greater in the alcohol than control decidua at 6 hours after injection (P < .01). This effect persisted at 24 hours (P < .01). There was no difference in c-fos expression in embryos whose mothers received alcohol versus control after 6 hours (P = .12) or 24 hours (P = .89). CONCLUSION: Alcohol administration during pregnancy results in differential gene expression in the stress signal pathway, particularly in c-fos. C-fos expression in the decidua increases from 6 to 24 hours after alcohol injection, but does not change in the embryo, which may contribute to alcohol-induced damage in FAS.

AB - OBJECTIVE: Fetal alcohol syndrome (FAS) results in stillbirth, fetal growth restriction, and mental retardation with injury attributed to oxidative stress. Our objective was to identify signal transduction pathways expressed in a model of FAS and to quantify expression of c-fos, a gene in the stress signal pathway. STUDY DESIGN: Timed, pregnant C57BI6/J mice were injected on E8 with saline solution or alcohol. RNA was extracted from decidua and embryo 6 and 24 hours later. Microarray analysis was used to screen gene pathways. Differential gene expression was confirmed using real-time polymerase chain reaction with results presented as the ratio of c-fos concentration to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). RESULTS: Differential gene expression between alcohol and control was noted for stress signal pathway genes including c-fos. Real-time polymerase chain reaction demonstrated that c-fos messenger RNA expression was greater in the alcohol than control decidua at 6 hours after injection (P < .01). This effect persisted at 24 hours (P < .01). There was no difference in c-fos expression in embryos whose mothers received alcohol versus control after 6 hours (P = .12) or 24 hours (P = .89). CONCLUSION: Alcohol administration during pregnancy results in differential gene expression in the stress signal pathway, particularly in c-fos. C-fos expression in the decidua increases from 6 to 24 hours after alcohol injection, but does not change in the embryo, which may contribute to alcohol-induced damage in FAS.

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