Differential expression of Orexin receptors 1 and 2 in the rat brain

Jacob N. Marcus, Carl J. Aschkenasi, Charlotte E. Lee, Richard M. Chemelli, Clifford B. Saper, Masashi Yanagisawa, Joel K Elmquist

Research output: Contribution to journalArticle

1113 Citations (Scopus)

Abstract

Orexins (hypocretins) are neuropeptides synthesized in the central nervous system exclusively by neurons of the lateral hypothalamus. Orexin-containing neurons have widespread projections and have been implicated in complex physiological functions including feeding behavior, sleep states, neuroendocrine function, and autonomic control. Two orexin receptors (OX1R and OX2R) have been identified, with distinct expression patterns throughout the brain, but a systematic examination of orexin receptor expression in the brain has not appeared. We used in situ hybridization histochemistry to examine the patterns of expression of mRNA for both orexin receptors throughout the brain. OX1R mRNA was observed in many brain regions including the prefrontal and infralimbic cortex, hippocampus, paraventricular thalamic nucleus, ventromedial hypothalamic nucleus, dorsal raphe nucleus, and locus coeruleus. OX2R mRNA was prominent in a complementary distribution including the cerebral cortex, septal nuclei, hippocampus, medial thalamic groups, raphe nuclei, and many hypothalamic nuclei including the tuberomammillary nucleus, dorsomedial nucleus, paraventricular nucleus, and ventral premammillary nucleus. The differential distribution of orexin receptors is consistent with the proposed multifaceted roles of orexin in regulating homeostasis and may explain the unique role of the OX2R receptor in regulating sleep state stability.

Original languageEnglish (US)
Pages (from-to)6-25
Number of pages20
JournalJournal of Comparative Neurology
Volume435
Issue number1
DOIs
StatePublished - Jun 18 2001

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Orexin Receptors
Lateral Hypothalamic Area
Mediodorsal Thalamic Nucleus
Brain
Messenger RNA
Hippocampus
Sleep
Midline Thalamic Nuclei
Ventromedial Hypothalamic Nucleus
Posterior Hypothalamus
Neurons
Septal Nuclei
Raphe Nuclei
Locus Coeruleus
Paraventricular Hypothalamic Nucleus
Feeding Behavior
Prefrontal Cortex
Neuropeptides
Cerebral Cortex
In Situ Hybridization

Keywords

  • Feeding
  • Hypocretin
  • Hypothalamus
  • Narcolepsy
  • Neuropeptide
  • Sleep

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential expression of Orexin receptors 1 and 2 in the rat brain. / Marcus, Jacob N.; Aschkenasi, Carl J.; Lee, Charlotte E.; Chemelli, Richard M.; Saper, Clifford B.; Yanagisawa, Masashi; Elmquist, Joel K.

In: Journal of Comparative Neurology, Vol. 435, No. 1, 18.06.2001, p. 6-25.

Research output: Contribution to journalArticle

Marcus, Jacob N. ; Aschkenasi, Carl J. ; Lee, Charlotte E. ; Chemelli, Richard M. ; Saper, Clifford B. ; Yanagisawa, Masashi ; Elmquist, Joel K. / Differential expression of Orexin receptors 1 and 2 in the rat brain. In: Journal of Comparative Neurology. 2001 ; Vol. 435, No. 1. pp. 6-25.
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