Differential expression of the B cell-restricted molecule CD22 on neonatal B lymphocytes depending upon antigen stimulation

Dorothee Viemann, Peter Schlenke, Hans Jörg Hammers, Holger Kirchner, Andrea Kruse

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Newborns respond poorly to certain antigens and produce mainly IgM antibodies. By flow cytometry we analyzed on neonatal and adult B cells the expression of CD22, a B cell receptor (BCR)-associated membrane molecule, known as negative modulator of BCR signaling. After T cell-independent (TI-)stimulation with anti-μ F(ab')2 fragments we found a dramatic decrease in the percentage of neonatal CD22+ B cells and CD22 mean fluorescence intensity (MFI) shift, whereas adult B cells remained unaffected. Survival and proliferation rates of neonatal B cells were higher compared to adult B cells whereas the degrees of apoptosis and necrosis were comparable. Surprisingly, after stimulation with lower doses of anti-μ apoptosis as well as proliferation increased significantly in contrast to adult B cells. T cell-dependent (TD)-stimulation with anti-CD40 monoclonal antibody and IL-4 resulted in a dramatic increase in the percentage of CD22+ neonatal B cells in contrast to unaffected adult B cells. CD22 MFI shifts showed no significant changes, respectively. The survival rate was higher for adult B cells, whereas apoptosis and cell death were comparable. These results suggest that TI antigens lower the neonatal BCR signaling threshold via down-regulation of CD22, resulting in hyperresponsive B cells apt to premature apoptosis. On the other hand, up-regulation of CD22 after TD stimulation may allow increased inhibiting influence of CD22 on neonatal BCR signaling, impairing B cell activation and differentiation.

Original languageEnglish (US)
Pages (from-to)550-559
Number of pages10
JournalEuropean Journal of Immunology
Volume30
Issue number2
DOIs
StatePublished - Feb 28 2000

Keywords

  • Apoptosis
  • B cell activation
  • CD22
  • Cord blood

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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