Differential glycosylation regulates processing of lipoprotein receptors by γ-secretase

Petra May, Hans H. Bock, Johannes Nimpf, Joachim Herz

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The low density lipoprotein (LDL) receptor-related protein I (LRP1) belongs to a growing number of cell surface proteins that undergo regulated proteolytic processing that culminates in the release of their intracellular domain (ICD) by the intramembranous protease γ-secretase. Here we show that LRP1 is differentially glycosylated in a tissue-specific manner and that carbohydrate addition reduces proteolytic cleavage of the extracellular domain and, concomitantly, ICD release. The apolipoprotein E (apoE) receptor-2 (apoER2), another member of the LDL receptor family with functions in cellular signal transmission, also undergoes sequential proteolytic processing, resulting in intracellular domain release into the cytoplasm. The penultimate processing step also involves cleavage of the apoER2 extracellular domain. The rate at which this cleavage step occurs is determined by the glycosylation state of the receptor, which in turn is regulated by the alternative splicing of an exon encoding several O-linked sugar attachment sites. These findings suggest a role for differential and tissue-specific glycosylation as a physiological switch that modulates the diverse biological functions of these receptors in a cell-type specific manner.

Original languageEnglish (US)
Pages (from-to)37386-37392
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number39
DOIs
StatePublished - Sep 26 2003

Fingerprint

Lipoprotein Receptors
Glycosylation
Amyloid Precursor Protein Secretases
LDL Receptors
LDL-Receptor Related Proteins
Low Density Lipoprotein Receptor-Related Protein-1
Alternative Splicing
Processing
Tissue
Exons
Membrane Proteins
Cytoplasm
Peptide Hydrolases
Carbohydrates
Sugars
Switches
Proteins
low density lipoprotein receptor-related protein 8

ASJC Scopus subject areas

  • Biochemistry

Cite this

Differential glycosylation regulates processing of lipoprotein receptors by γ-secretase. / May, Petra; Bock, Hans H.; Nimpf, Johannes; Herz, Joachim.

In: Journal of Biological Chemistry, Vol. 278, No. 39, 26.09.2003, p. 37386-37392.

Research output: Contribution to journalArticle

May, Petra ; Bock, Hans H. ; Nimpf, Johannes ; Herz, Joachim. / Differential glycosylation regulates processing of lipoprotein receptors by γ-secretase. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 39. pp. 37386-37392.
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