Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome

Junjun Gao, Kangseok Lee, Meng Zhao, Ji Qiu, Xiaoming Zhan, Ankur Saxena, Christopher J. Moore, Stanley N. Cohen, George Georgiou

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

In Escherichia coli the initial step in the processing or decay of many messenger and structural RNAs is mediated by the endonuclease RNase E, which forms the core of a large RNA-catalysis machine termed the degradosome. Previous experiments have identified a protein that globally modulates RNA abundance by binding to RNase E and regulating its endonucleolytic activity. Here we report the discovery of RraB, which interacts with a different site on RNase E and interferes with cleavage of a different set of transcripts. We show that expression of RraA or RraB in vivo is accompanied by dramatic, distinct, and inhibitor-specific changes in degradosome composition - and that these are in turn associated with alterations in RNA decay and global transcript abundance profiles that are dissimilar to the profile observed during simple RNase E deficiency. Our results reveal the existence of endonuclease binding proteins that modulate the remodelling of degradosome composition in bacteria and argue that such degradosome remodelling is a mechanism for the differential regulation of RNA cleavages in E. coli.

Original languageEnglish (US)
Pages (from-to)394-406
Number of pages13
JournalMolecular Microbiology
Volume61
Issue number2
DOIs
StatePublished - Jul 2006

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Escherichia coli
Messenger RNA
Endonucleases
Proteins
RNA Cleavage
RNA
RNA Stability
Catalysis
Carrier Proteins
Bacteria
ribonuclease E
degradosome

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome. / Gao, Junjun; Lee, Kangseok; Zhao, Meng; Qiu, Ji; Zhan, Xiaoming; Saxena, Ankur; Moore, Christopher J.; Cohen, Stanley N.; Georgiou, George.

In: Molecular Microbiology, Vol. 61, No. 2, 07.2006, p. 394-406.

Research output: Contribution to journalArticle

Gao, Junjun ; Lee, Kangseok ; Zhao, Meng ; Qiu, Ji ; Zhan, Xiaoming ; Saxena, Ankur ; Moore, Christopher J. ; Cohen, Stanley N. ; Georgiou, George. / Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome. In: Molecular Microbiology. 2006 ; Vol. 61, No. 2. pp. 394-406.
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