TY - JOUR
T1 - Differential regulation of rat glomerular and proximal tubular renin mRNA following uninephrectomy
AU - Tank, Julia E.
AU - Moe, Orson W.
AU - Star, Robert A.
AU - Henrich, William L.
PY - 1996
Y1 - 1996
N2 - role in the renal adaptations to reduced renal mass, but earlier work has shown that plasma renin activity (PRA) does not increase in this setting. To examine this paradox, we studied the effect of uninephrectomy (UNX) on circulating, juxtaglomerular, glomerular, and proximal tubular (PT) renin. PRA was unchanged 2 wk following UNX and fell slightly at 6 wk. Single kidney renin secretory capacity and cortical renin mRNA, reflecting juxtaglomerular renin, were unchanged at 2 and 6 wk. With quantitative competitive reverse transcription-polymerase chain reaction, renin mRNA in microdissected glomeruli and PT were dramatically increased 2 wk post-UNX (for glomeruli: sham, 1.2 ± 0.3, vs. UNX, 8.8 ± 1.9 × 105 copies/glomerulus; for PT: sham, 4.6 ± 0.9, vs. UNX, 17.7 ± 5.1 × 103 copies/mm). By 6 wk, glomerular renin was unchanged, and PT renin mRNA was markedly suppressed (for glomeruli: sham, 2.9 ± 1.2, vs. UNX, 4.2 ± 1.1 × 105 copies/glomerulus; for PT: sham, 7.5 ± 2.1, vs. UNX, 1.0 ± 0.3 × 103 copies/mm). These results demonstrate differential regulation of the circulating, juxtaglomerular, glomerular, and PT renin systems. Early activation of glomerular and PT renin may result in increased local generation of angiotensin and thereby affect renal structural and functional adaptations following UNX. polymerase chain reaction; glomerulus; proximal tubule; kidney; angiotensin II.
AB - role in the renal adaptations to reduced renal mass, but earlier work has shown that plasma renin activity (PRA) does not increase in this setting. To examine this paradox, we studied the effect of uninephrectomy (UNX) on circulating, juxtaglomerular, glomerular, and proximal tubular (PT) renin. PRA was unchanged 2 wk following UNX and fell slightly at 6 wk. Single kidney renin secretory capacity and cortical renin mRNA, reflecting juxtaglomerular renin, were unchanged at 2 and 6 wk. With quantitative competitive reverse transcription-polymerase chain reaction, renin mRNA in microdissected glomeruli and PT were dramatically increased 2 wk post-UNX (for glomeruli: sham, 1.2 ± 0.3, vs. UNX, 8.8 ± 1.9 × 105 copies/glomerulus; for PT: sham, 4.6 ± 0.9, vs. UNX, 17.7 ± 5.1 × 103 copies/mm). By 6 wk, glomerular renin was unchanged, and PT renin mRNA was markedly suppressed (for glomeruli: sham, 2.9 ± 1.2, vs. UNX, 4.2 ± 1.1 × 105 copies/glomerulus; for PT: sham, 7.5 ± 2.1, vs. UNX, 1.0 ± 0.3 × 103 copies/mm). These results demonstrate differential regulation of the circulating, juxtaglomerular, glomerular, and PT renin systems. Early activation of glomerular and PT renin may result in increased local generation of angiotensin and thereby affect renal structural and functional adaptations following UNX. polymerase chain reaction; glomerulus; proximal tubule; kidney; angiotensin II.
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U2 - 10.1152/ajprenal.1996.270.5.f776
DO - 10.1152/ajprenal.1996.270.5.f776
M3 - Article
C2 - 8928838
AN - SCOPUS:0029950246
SN - 0002-9513
VL - 270
SP - F776-F783
JO - American Journal of Physiology
JF - American Journal of Physiology
IS - 5 PART 2
ER -