Differential regulation of rat glomerular and proximal tubular renin mRNA following uninephrectomy

Julia E. Tank, Orson W. Moe, Robert A. Star, William L. Henrich

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

role in the renal adaptations to reduced renal mass, but earlier work has shown that plasma renin activity (PRA) does not increase in this setting. To examine this paradox, we studied the effect of uninephrectomy (UNX) on circulating, juxtaglomerular, glomerular, and proximal tubular (PT) renin. PRA was unchanged 2 wk following UNX and fell slightly at 6 wk. Single kidney renin secretory capacity and cortical renin mRNA, reflecting juxtaglomerular renin, were unchanged at 2 and 6 wk. With quantitative competitive reverse transcription-polymerase chain reaction, renin mRNA in microdissected glomeruli and PT were dramatically increased 2 wk post-UNX (for glomeruli: sham, 1.2 ± 0.3, vs. UNX, 8.8 ± 1.9 × 105 copies/glomerulus; for PT: sham, 4.6 ± 0.9, vs. UNX, 17.7 ± 5.1 × 103 copies/mm). By 6 wk, glomerular renin was unchanged, and PT renin mRNA was markedly suppressed (for glomeruli: sham, 2.9 ± 1.2, vs. UNX, 4.2 ± 1.1 × 105 copies/glomerulus; for PT: sham, 7.5 ± 2.1, vs. UNX, 1.0 ± 0.3 × 103 copies/mm). These results demonstrate differential regulation of the circulating, juxtaglomerular, glomerular, and PT renin systems. Early activation of glomerular and PT renin may result in increased local generation of angiotensin and thereby affect renal structural and functional adaptations following UNX. polymerase chain reaction; glomerulus; proximal tubule; kidney; angiotensin II.

Original languageEnglish (US)
JournalAmerican Journal of Physiology
Volume270
Issue number5 PART 2
StatePublished - 1996

Fingerprint

Renin
Messenger RNA
Kidney
Polymerase Chain Reaction
Angiotensins
Angiotensin II
Reverse Transcription

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

Differential regulation of rat glomerular and proximal tubular renin mRNA following uninephrectomy. / Tank, Julia E.; Moe, Orson W.; Star, Robert A.; Henrich, William L.

In: American Journal of Physiology, Vol. 270, No. 5 PART 2, 1996.

Research output: Contribution to journalArticle

@article{a21f8e9ae2d54bffa67cc5b8d02aae5d,
title = "Differential regulation of rat glomerular and proximal tubular renin mRNA following uninephrectomy",
abstract = "role in the renal adaptations to reduced renal mass, but earlier work has shown that plasma renin activity (PRA) does not increase in this setting. To examine this paradox, we studied the effect of uninephrectomy (UNX) on circulating, juxtaglomerular, glomerular, and proximal tubular (PT) renin. PRA was unchanged 2 wk following UNX and fell slightly at 6 wk. Single kidney renin secretory capacity and cortical renin mRNA, reflecting juxtaglomerular renin, were unchanged at 2 and 6 wk. With quantitative competitive reverse transcription-polymerase chain reaction, renin mRNA in microdissected glomeruli and PT were dramatically increased 2 wk post-UNX (for glomeruli: sham, 1.2 ± 0.3, vs. UNX, 8.8 ± 1.9 × 105 copies/glomerulus; for PT: sham, 4.6 ± 0.9, vs. UNX, 17.7 ± 5.1 × 103 copies/mm). By 6 wk, glomerular renin was unchanged, and PT renin mRNA was markedly suppressed (for glomeruli: sham, 2.9 ± 1.2, vs. UNX, 4.2 ± 1.1 × 105 copies/glomerulus; for PT: sham, 7.5 ± 2.1, vs. UNX, 1.0 ± 0.3 × 103 copies/mm). These results demonstrate differential regulation of the circulating, juxtaglomerular, glomerular, and PT renin systems. Early activation of glomerular and PT renin may result in increased local generation of angiotensin and thereby affect renal structural and functional adaptations following UNX. polymerase chain reaction; glomerulus; proximal tubule; kidney; angiotensin II.",
author = "Tank, {Julia E.} and Moe, {Orson W.} and Star, {Robert A.} and Henrich, {William L.}",
year = "1996",
language = "English (US)",
volume = "270",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "5 PART 2",

}

TY - JOUR

T1 - Differential regulation of rat glomerular and proximal tubular renin mRNA following uninephrectomy

AU - Tank, Julia E.

AU - Moe, Orson W.

AU - Star, Robert A.

AU - Henrich, William L.

PY - 1996

Y1 - 1996

N2 - role in the renal adaptations to reduced renal mass, but earlier work has shown that plasma renin activity (PRA) does not increase in this setting. To examine this paradox, we studied the effect of uninephrectomy (UNX) on circulating, juxtaglomerular, glomerular, and proximal tubular (PT) renin. PRA was unchanged 2 wk following UNX and fell slightly at 6 wk. Single kidney renin secretory capacity and cortical renin mRNA, reflecting juxtaglomerular renin, were unchanged at 2 and 6 wk. With quantitative competitive reverse transcription-polymerase chain reaction, renin mRNA in microdissected glomeruli and PT were dramatically increased 2 wk post-UNX (for glomeruli: sham, 1.2 ± 0.3, vs. UNX, 8.8 ± 1.9 × 105 copies/glomerulus; for PT: sham, 4.6 ± 0.9, vs. UNX, 17.7 ± 5.1 × 103 copies/mm). By 6 wk, glomerular renin was unchanged, and PT renin mRNA was markedly suppressed (for glomeruli: sham, 2.9 ± 1.2, vs. UNX, 4.2 ± 1.1 × 105 copies/glomerulus; for PT: sham, 7.5 ± 2.1, vs. UNX, 1.0 ± 0.3 × 103 copies/mm). These results demonstrate differential regulation of the circulating, juxtaglomerular, glomerular, and PT renin systems. Early activation of glomerular and PT renin may result in increased local generation of angiotensin and thereby affect renal structural and functional adaptations following UNX. polymerase chain reaction; glomerulus; proximal tubule; kidney; angiotensin II.

AB - role in the renal adaptations to reduced renal mass, but earlier work has shown that plasma renin activity (PRA) does not increase in this setting. To examine this paradox, we studied the effect of uninephrectomy (UNX) on circulating, juxtaglomerular, glomerular, and proximal tubular (PT) renin. PRA was unchanged 2 wk following UNX and fell slightly at 6 wk. Single kidney renin secretory capacity and cortical renin mRNA, reflecting juxtaglomerular renin, were unchanged at 2 and 6 wk. With quantitative competitive reverse transcription-polymerase chain reaction, renin mRNA in microdissected glomeruli and PT were dramatically increased 2 wk post-UNX (for glomeruli: sham, 1.2 ± 0.3, vs. UNX, 8.8 ± 1.9 × 105 copies/glomerulus; for PT: sham, 4.6 ± 0.9, vs. UNX, 17.7 ± 5.1 × 103 copies/mm). By 6 wk, glomerular renin was unchanged, and PT renin mRNA was markedly suppressed (for glomeruli: sham, 2.9 ± 1.2, vs. UNX, 4.2 ± 1.1 × 105 copies/glomerulus; for PT: sham, 7.5 ± 2.1, vs. UNX, 1.0 ± 0.3 × 103 copies/mm). These results demonstrate differential regulation of the circulating, juxtaglomerular, glomerular, and PT renin systems. Early activation of glomerular and PT renin may result in increased local generation of angiotensin and thereby affect renal structural and functional adaptations following UNX. polymerase chain reaction; glomerulus; proximal tubule; kidney; angiotensin II.

UR - http://www.scopus.com/inward/record.url?scp=0029950246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029950246&partnerID=8YFLogxK

M3 - Article

C2 - 8928838

AN - SCOPUS:0029950246

VL - 270

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 5 PART 2

ER -