Differential regulation of Rho GTPases by β1 and β3 integrins: The role of an extracellular domain of integrin in intracellular signaling

Hui Miao, Song Li, Ying Li Hu, Suli Yuan, Yihua Zhao, Benjamin P C Chen, Wilma Puzon-McLaughlin, Takehiko Tarui, John Y J Shyy, Yoshikazu Takada, Shunichi Usami, Shu Chien

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Integrins mediate cell adhesion and signal transduction at focal adhesions. Here we investigate the roles of integrin β subunits in the regulation of actin cytoskeletal structure and the activities of Rho and Rac. The overexpression of β3 integrin in Chinese hamster ovary cells enhances Rho activity and stress fiber formation, whereas the overexpression of β1 integrin increases Rac activity and lamellipodia formation. The overexpression of a mutant β1-3-1 integrin, in which the extracellular I-domain-like sequence of β1 integrin has been replaced with the corresponding sequence of β3 integrin, also enhances Rho activity and the formation of stress fibers. Our results demonstrate that β1 and β3 integrins differentially regulate the activities of Rho family GTPases and that the extracellular domains of integrin β subunits play a critical role in transducing the extracellular ligand-binding information into specific intracellular signaling events.

Original languageEnglish (US)
Pages (from-to)2199-2206
Number of pages8
JournalJournal of Cell Science
Volume115
Issue number10
StatePublished - May 15 2002

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rho GTP-Binding Proteins
Integrins
Stress Fibers
Pseudopodia
Focal Adhesions
Cricetulus
Cell Adhesion
Actins
Ovary
Signal Transduction
Ligands

Keywords

  • Extracellular domain
  • Integrin
  • Signal transduction
  • Small GTPase

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Differential regulation of Rho GTPases by β1 and β3 integrins : The role of an extracellular domain of integrin in intracellular signaling. / Miao, Hui; Li, Song; Hu, Ying Li; Yuan, Suli; Zhao, Yihua; Chen, Benjamin P C; Puzon-McLaughlin, Wilma; Tarui, Takehiko; Shyy, John Y J; Takada, Yoshikazu; Usami, Shunichi; Chien, Shu.

In: Journal of Cell Science, Vol. 115, No. 10, 15.05.2002, p. 2199-2206.

Research output: Contribution to journalArticle

Miao, H, Li, S, Hu, YL, Yuan, S, Zhao, Y, Chen, BPC, Puzon-McLaughlin, W, Tarui, T, Shyy, JYJ, Takada, Y, Usami, S & Chien, S 2002, 'Differential regulation of Rho GTPases by β1 and β3 integrins: The role of an extracellular domain of integrin in intracellular signaling', Journal of Cell Science, vol. 115, no. 10, pp. 2199-2206.
Miao, Hui ; Li, Song ; Hu, Ying Li ; Yuan, Suli ; Zhao, Yihua ; Chen, Benjamin P C ; Puzon-McLaughlin, Wilma ; Tarui, Takehiko ; Shyy, John Y J ; Takada, Yoshikazu ; Usami, Shunichi ; Chien, Shu. / Differential regulation of Rho GTPases by β1 and β3 integrins : The role of an extracellular domain of integrin in intracellular signaling. In: Journal of Cell Science. 2002 ; Vol. 115, No. 10. pp. 2199-2206.
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AU - Puzon-McLaughlin, Wilma

AU - Tarui, Takehiko

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