Differential roles of the dopamine 1-class receptors, D1R and D5R, in hippocampal dependent memory

Joshua Sariñana, Takashi Kitamura, Patrik Künzler, Lisa Sultzman, Susumu Tonegawa

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Activation of the hippocampal dopamine 1-class receptors (D1R and D5R) are implicated in contextual fear conditioning (CFC). However, the specific role of the D1R versus D5R in hippocampal dependent CFC has not been investigated. Generation of D1R- and D5R-specific in situ hybridization probes showed that D1R and D5R mRNA expression was greatest in the dentate gyrus (DG) of the hippocampus. To identify the role of each receptor in CFC we generated spatially restricted KO mice that lack either the D1R or D5R in DG granule cells. DG D1R KOs displayed significant fear memory deficits, whereas DG D5R KOs did not. Furthermore, D1R KOs but not D5R KOs, exhibited generalized fear between two similar but different contexts. In the familiar home cage context, c-Fos expression was relatively low in the DG of control mice, and it increased upon exposure to a novel context. This level of c-Fos expression in the DG did not further increase when a footshock was delivered in the novel context. In DG D1R KOs, DG c-Fos levels in the home cage was higher than that of the control mice, but it did not further increase upon exposure to a novel context and remained at the same level upon a shock delivery. In contrast, the levels of DG c-Fos expression was unaffected by the deletion of DG D5R neither in the home cage nor upon a shock delivery. These results suggest that DG D1Rs, but not D5Rs, contribute to the formation of distinct contextual representations of novel environments.

Original languageEnglish (US)
Pages (from-to)8245-8250
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number22
DOIs
StatePublished - Jun 3 2014

Fingerprint

Dentate Gyrus
Dopamine
Fear
Shock
Parahippocampal Gyrus
Memory Disorders
In Situ Hybridization
Messenger RNA

Keywords

  • Dopamine 1 receptor
  • Dopamine 5 receptor
  • Memory generalization
  • Pavlovian fear conditioning

ASJC Scopus subject areas

  • General

Cite this

Differential roles of the dopamine 1-class receptors, D1R and D5R, in hippocampal dependent memory. / Sariñana, Joshua; Kitamura, Takashi; Künzler, Patrik; Sultzman, Lisa; Tonegawa, Susumu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 22, 03.06.2014, p. 8245-8250.

Research output: Contribution to journalArticle

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