Differential subcellular membrane recruitment of Src may specify its downstream signalling

Philippe de Diesbach, Thierry Medts, Sarah Carpentier, Ludovic D'Auria, Patrick Van Der Smissen, Anna Platek, Marcel Mettlen, Adrian Caplanusi, Marie France van den Hove, Donatienne Tyteca, Pierre J. Courtoy

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Most Src family members are diacylated and constitutively associate with membrane "lipid rafts" that coordinate signalling. Whether the monoacylated Src, frequently hyperactive in carcinomas, also localizes at "rafts" remains controversial. Using polarized MDCK cells expressing the thermosensitive v-Src/tsLA31 variant, we here addressed how Src tyrosine-kinase activation may impact on its (i) membrane recruitment, in particular to "lipid rafts"; (ii) subcellular localization; and (iii) signalling. The kinetics of Src-kinase thermoactivation correlated with its recruitment from the cytosol to sedimentable membranes where Src largely resisted solubilisation by non-ionic detergents at 4 °C and floated into sucrose density gradients like caveolin-1 and flotillin-2, i.e. "lipid rafts". By immunofluorescence, activated Src showed a dual localization, at apical endosomes/macropinosomes and at the apical plasma membrane. The plasma membrane Src pool did not colocalize with caveolin-1 and flotillin-2, but extensively overlapped GM1 labelling by cholera toxin. Severe (∼ 70%) cholesterol extraction with methyl-β-cyclodextrin (MβCD) did not abolish "rafts" floatation, but strongly decreased Src association with floating "rafts" and abolished its localization at the apical plasma membrane. Src activation independently activated first the MAP-kinase - ERK1/2 pathway, then the PI3-kinase - Akt pathway. MAP-kinase - ERK1/2 activation was insensitive to MβCD, which suppressed Akt phosphorylation and apical endocytosis induced by Src, both depending on the PI3-kinase pathway. We therefore suggest that activated Src is recruited at two membrane compartments, allowing differential signalling, first via ERK1/2 at "non-raft" domains on endosomes, then via PI3-kinase-Akt on a distinct set of "rafts" at the apical plasma membrane. Whether this model is applicable to c-Src remains to be examined.

Original languageEnglish (US)
Pages (from-to)1465-1479
Number of pages15
JournalExperimental Cell Research
Volume314
Issue number7
DOIs
StatePublished - Apr 15 2008

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Phosphatidylinositol 3-Kinases
Cell Membrane
Caveolin 1
Membranes
src-Family Kinases
Endosomes
Mitogen-Activated Protein Kinase 1
Cyclodextrins
Lipids
Madin Darby Canine Kidney Cells
MAP Kinase Signaling System
Cholera Toxin
Membrane Lipids
Endocytosis
Detergents
Cytosol
Fluorescent Antibody Technique
Sucrose
Cholesterol
Phosphorylation

Keywords

  • Endosomes
  • MAP-kinase
  • PI3-kinase
  • Plasma membrane
  • Rafts
  • Src

ASJC Scopus subject areas

  • Cell Biology

Cite this

de Diesbach, P., Medts, T., Carpentier, S., D'Auria, L., Van Der Smissen, P., Platek, A., ... Courtoy, P. J. (2008). Differential subcellular membrane recruitment of Src may specify its downstream signalling. Experimental Cell Research, 314(7), 1465-1479. https://doi.org/10.1016/j.yexcr.2008.01.015

Differential subcellular membrane recruitment of Src may specify its downstream signalling. / de Diesbach, Philippe; Medts, Thierry; Carpentier, Sarah; D'Auria, Ludovic; Van Der Smissen, Patrick; Platek, Anna; Mettlen, Marcel; Caplanusi, Adrian; van den Hove, Marie France; Tyteca, Donatienne; Courtoy, Pierre J.

In: Experimental Cell Research, Vol. 314, No. 7, 15.04.2008, p. 1465-1479.

Research output: Contribution to journalArticle

de Diesbach, P, Medts, T, Carpentier, S, D'Auria, L, Van Der Smissen, P, Platek, A, Mettlen, M, Caplanusi, A, van den Hove, MF, Tyteca, D & Courtoy, PJ 2008, 'Differential subcellular membrane recruitment of Src may specify its downstream signalling', Experimental Cell Research, vol. 314, no. 7, pp. 1465-1479. https://doi.org/10.1016/j.yexcr.2008.01.015
de Diesbach P, Medts T, Carpentier S, D'Auria L, Van Der Smissen P, Platek A et al. Differential subcellular membrane recruitment of Src may specify its downstream signalling. Experimental Cell Research. 2008 Apr 15;314(7):1465-1479. https://doi.org/10.1016/j.yexcr.2008.01.015
de Diesbach, Philippe ; Medts, Thierry ; Carpentier, Sarah ; D'Auria, Ludovic ; Van Der Smissen, Patrick ; Platek, Anna ; Mettlen, Marcel ; Caplanusi, Adrian ; van den Hove, Marie France ; Tyteca, Donatienne ; Courtoy, Pierre J. / Differential subcellular membrane recruitment of Src may specify its downstream signalling. In: Experimental Cell Research. 2008 ; Vol. 314, No. 7. pp. 1465-1479.
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AU - Platek, Anna

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