Differential susceptibility of neonatal and adult murine spleen cells to in vitro induction of B cell tolerance

J. C. Cambier, J. R. Kettman, E. S. Vitetta, J. W. Uhr

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The relative susceptibility of neonatal and adult murine splenocytes to induction of B cell tolerance was studied in vitro. Adult cells required approximately 1,000 fold more trinitrophenyl human gamma globulin to be rendered tolerant than did cells from 9 to 12 day old neonates. The potential effects of suppressor T cells were excluded by pretreating the cultured B cells with anti Thy 1 and C' and the helper T cells with anti Ly 2.2 and C'. The possible role of cell surface immunoglobulin isotypes in contributing to this observed difference is discussed.

Original languageEnglish (US)
Pages (from-to)293-297
Number of pages5
JournalJournal of Experimental Medicine
Volume144
Issue number1
StatePublished - 1976

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B-Lymphocytes
Spleen
B-Cell Antigen Receptors
Immunoglobulin Isotypes
gamma-Globulins
Helper-Inducer T-Lymphocytes
Cultured Cells
T-Lymphocytes
In Vitro Techniques
anti-Thy antibody

ASJC Scopus subject areas

  • Immunology

Cite this

Differential susceptibility of neonatal and adult murine spleen cells to in vitro induction of B cell tolerance. / Cambier, J. C.; Kettman, J. R.; Vitetta, E. S.; Uhr, J. W.

In: Journal of Experimental Medicine, Vol. 144, No. 1, 1976, p. 293-297.

Research output: Contribution to journalArticle

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