Abstract
The cDNA clones for two distinct mRNAs encoding one of the two known isoforms of chicken smooth muscle/nonmuscle myosin regulatory light chain were isolated. The nucleotide sequences of these cDNAs were very similar to each other (99% nucleotide identities) in the 516 bp translated regions and in the first 33 bp of the 3′ noncoding regions, whereas the rest of the 3′ noncoding regions and the 5′ noncoding regions had no significant similarity. Genomic Southern blot analysis showed that these two mRNAs were encoded in two individual genes. Whereas these two genes encoded almost identical polypeptides with only one conservative substitution of amino acid residues, expression of the mRNAs was differentially regulated both at the transcriptional and translational levels in various tissues of the chicken.
Original language | English (US) |
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Pages (from-to) | 197-202 |
Number of pages | 6 |
Journal | BBA - Gene Structure and Expression |
Volume | 1130 |
Issue number | 2 |
DOIs | |
State | Published - Mar 24 1992 |
Keywords
- Myosin light chain
- Nonmuscle myosin
- Smooth muscle
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics