Differential tissue expression of multiple genes for chicken smooth muscle/nonmuscle myosin regulatory light chains

Akihiro Inoue, Masashi Yanagisawa, Tomoh Masaki

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The cDNA clones for two distinct mRNAs encoding one of the two known isoforms of chicken smooth muscle/nonmuscle myosin regulatory light chain were isolated. The nucleotide sequences of these cDNAs were very similar to each other (99% nucleotide identities) in the 516 bp translated regions and in the first 33 bp of the 3′ noncoding regions, whereas the rest of the 3′ noncoding regions and the 5′ noncoding regions had no significant similarity. Genomic Southern blot analysis showed that these two mRNAs were encoded in two individual genes. Whereas these two genes encoded almost identical polypeptides with only one conservative substitution of amino acid residues, expression of the mRNAs was differentially regulated both at the transcriptional and translational levels in various tissues of the chicken.

Original languageEnglish (US)
Pages (from-to)197-202
Number of pages6
JournalBBA - Gene Structure and Expression
Volume1130
Issue number2
DOIs
StatePublished - Mar 24 1992

Keywords

  • Myosin light chain
  • Nonmuscle myosin
  • Smooth muscle

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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