TY - JOUR
T1 - Differential transmission of actin motion within focal adhesions
AU - Hu, Ke
AU - Ji, Lin
AU - Applegate, Kathryn T.
AU - Danuser, Gaudenz
AU - Waterman-Storer, Clare M.
PY - 2007/1/5
Y1 - 2007/1/5
N2 - Cell migration requires the transmission of motion generated in the actin cytoskeleton to the extracellular environment through a complex assembly of proteins in focal adhesions. We developed correlational fluorescent speckle microscopy to measure the coupling of focal-adhesion proteins to actin filaments. Different classes of focal-adhesion structural and regulatory molecules exhibited varying degrees of correlated motions with actin filaments, indicating hierarchical transmission of actin motion through focal adhesions. Interactions between vinculin, talin, and actin filaments appear to constitute a slippage interface between the cytoskeleton and integrins, generating a molecular clutch that is regulated during the morphodynamic transitions of cell migration.
AB - Cell migration requires the transmission of motion generated in the actin cytoskeleton to the extracellular environment through a complex assembly of proteins in focal adhesions. We developed correlational fluorescent speckle microscopy to measure the coupling of focal-adhesion proteins to actin filaments. Different classes of focal-adhesion structural and regulatory molecules exhibited varying degrees of correlated motions with actin filaments, indicating hierarchical transmission of actin motion through focal adhesions. Interactions between vinculin, talin, and actin filaments appear to constitute a slippage interface between the cytoskeleton and integrins, generating a molecular clutch that is regulated during the morphodynamic transitions of cell migration.
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U2 - 10.1126/science.1135085
DO - 10.1126/science.1135085
M3 - Article
C2 - 17204653
AN - SCOPUS:33846697554
SN - 0036-8075
VL - 315
SP - 111
EP - 115
JO - Science
JF - Science
IS - 5808
ER -