Differentially expressed circulating LncRNAs and mRNA identified by microarray analysis in obese patients

Jia Sun, Yuting Ruan, Ming Wang, Rongping Chen, Na Yu, Lei Sun, Tiemin Liu, Hong Chen

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Circulating long non-coding RNAs (lncRNAs) serve as valuable biomarkers in a number of human diseases. However, lncRNA biomarkers have yet to be identified in obesity. We aim to characterize circulating lncRNA expression in obese and non-obese human subjects. First, we assessed the genome-wide circulating lncRNA expression profiles in blood from 3 obese and 3 non-obese human subjects. We found a significant decrease in circulating levels of three lncRNAs (lncRNA-p5549, lncRNA-p21015 and lncRNA-p19461) in obese human subjects only. Next, using RT-PCR we measured the expression levels of these three lncRNAs in 33 obese and 33 non-obese human subjects and found similar differences. Moreover, we found a negative correlation between circulating levels of these three lncRNAs and body mass index (BMI), waist circumference, waist to hip ratio and fasting insulin. There was also a significant negative correlation between expression of lncRNA-p19461 and homeostasis model assessment-estimated insulin resistance. Finally, we tested the circulating levels of these three lncRNAs in 8 obese human subjects after a 12-week diet-induced weight loss program. We found that only lncRNA-p19461 expression level significantly increased. In summary, circulating lncRNAs are deregulated in obesity. Weight loss-induced changes in this profile support this observation and suggest a potential mechanistic relevance.

Original languageEnglish (US)
Article number35421
JournalScientific Reports
Volume6
DOIs
StatePublished - Oct 21 2016

ASJC Scopus subject areas

  • General

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