Differentiation-dependent chromatin alterations precede and accompany transcription of immunoglobulin light chain genes

S. M. Rose, W. T. Garrard

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

We have studied the nature of chromatin alterations along immunoglobulin light chain (IgL) genes during B cell development using cultured murine cell lines. Employing a chromatin fractionation procedure on micrococcal nuclease-treated nuclei, we demonstrate that transcriptionally active κ IgL chromatin lacks a canonical nucleosomal repeat and exhibits a pronoduced association with insoluble nuclear material but is processed by nuclease to a soluble nucleosomal component that apparently lacks histone H1 and is enriched in high mobility group proteins. Of particular significance, utilizing a variant plasmacytoma cell line that has transcriptionally inactivated one κ allele via a promoter deletion, we demonstrate that transcription per se is not responsible for these novel alterations. Furthermore, we show that the chromatin encompassing germline (unrearranged) and transcriptionally silent λ IgL alleles in κ-producing plasmacytomas exhibit some of the same unusual properties that are displayed by κ alleles. Finally, we demonstrate that these alterations only occur in cell lines of the lymphocyte lineage that have progressed past the early pre-B cell stage; when inactive, both κ and λ IgL genes possess typical nucleosomal packaging and co-fractionate with histone H1-containing chromatin. These findings lead us to propose a model that predicts B cell stage-specific alternations in IgL chromatin prior to gene rearrangement and transcription.

Original languageEnglish (US)
Pages (from-to)8534-8544
Number of pages11
JournalJournal of Biological Chemistry
Volume259
Issue number13
StatePublished - 1984

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Immunoglobulin Light Chain Genes
Immunoglobulin Light Chains
Transcription
Chromatin
Genes
Cells
Plasmacytoma
Alleles
Cell Line
Histones
B-Lymphocytes
High Mobility Group Proteins
Micrococcal Nuclease
B-Lymphoid Precursor Cells
Lymphocytes
Gene Rearrangement
Product Packaging
Fractionation
Cultured Cells
Packaging

ASJC Scopus subject areas

  • Biochemistry

Cite this

Differentiation-dependent chromatin alterations precede and accompany transcription of immunoglobulin light chain genes. / Rose, S. M.; Garrard, W. T.

In: Journal of Biological Chemistry, Vol. 259, No. 13, 1984, p. 8534-8544.

Research output: Contribution to journalArticle

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