Differentiation-dependent expression of phosphatidylserine in mammalian plasma membranes: Quantitative assessment of outer-leaflet lipid by prothrombinase complex formation

J. Connor, C. Bucana, I. J. Fidler, A. J. Schroit

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Phosphatidylserine (PS) is asymmetrically distributed in mammalian cell membrane, being preferentially localized in the inner leaflet. Some studies have suggested that a disturbance in the normal asymmetric distribution of PS - e.g., PS exposure in the outer leaflet of the cell membrane, which can occur upon platelet activation as well as in certain pathologic red cells - serves as a potent procoagulant surface and as a single for triggering their recognition by macrophages. These studies suggest that the regulation of PS distribution in cell membranes may be critical in controlling coagulation and in determining the survival of pathologic cells in the circulation. In this paper we describe a sensitive technique, based on PS-dependent prothrombinase complex activity, for assessing the amount of PS on the external leaflet of intact viable cells. Our results indicate that tumorigenic, undifferentiated murine erythroleukemic cells express 7- to 8-fold more PS in their outer leaflet than do their differentiated, nontumorigenic counterparts. Increased expression of PS in the tumorigenic cells directly correlated with their ability to be recognized and bound by macrophages.

Original languageEnglish (US)
Pages (from-to)3184-3188
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number9
StatePublished - 1989

Fingerprint

Phosphatidylserines
Cell Membrane
Lipids
Macrophages
prothrombinase complex
Normal Distribution
Platelet Activation
Cell Survival

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{2e74da6e8e7d4160afd53d189ed8146f,
title = "Differentiation-dependent expression of phosphatidylserine in mammalian plasma membranes: Quantitative assessment of outer-leaflet lipid by prothrombinase complex formation",
abstract = "Phosphatidylserine (PS) is asymmetrically distributed in mammalian cell membrane, being preferentially localized in the inner leaflet. Some studies have suggested that a disturbance in the normal asymmetric distribution of PS - e.g., PS exposure in the outer leaflet of the cell membrane, which can occur upon platelet activation as well as in certain pathologic red cells - serves as a potent procoagulant surface and as a single for triggering their recognition by macrophages. These studies suggest that the regulation of PS distribution in cell membranes may be critical in controlling coagulation and in determining the survival of pathologic cells in the circulation. In this paper we describe a sensitive technique, based on PS-dependent prothrombinase complex activity, for assessing the amount of PS on the external leaflet of intact viable cells. Our results indicate that tumorigenic, undifferentiated murine erythroleukemic cells express 7- to 8-fold more PS in their outer leaflet than do their differentiated, nontumorigenic counterparts. Increased expression of PS in the tumorigenic cells directly correlated with their ability to be recognized and bound by macrophages.",
author = "J. Connor and C. Bucana and Fidler, {I. J.} and Schroit, {A. J.}",
year = "1989",
language = "English (US)",
volume = "86",
pages = "3184--3188",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "9",

}

TY - JOUR

T1 - Differentiation-dependent expression of phosphatidylserine in mammalian plasma membranes

T2 - Quantitative assessment of outer-leaflet lipid by prothrombinase complex formation

AU - Connor, J.

AU - Bucana, C.

AU - Fidler, I. J.

AU - Schroit, A. J.

PY - 1989

Y1 - 1989

N2 - Phosphatidylserine (PS) is asymmetrically distributed in mammalian cell membrane, being preferentially localized in the inner leaflet. Some studies have suggested that a disturbance in the normal asymmetric distribution of PS - e.g., PS exposure in the outer leaflet of the cell membrane, which can occur upon platelet activation as well as in certain pathologic red cells - serves as a potent procoagulant surface and as a single for triggering their recognition by macrophages. These studies suggest that the regulation of PS distribution in cell membranes may be critical in controlling coagulation and in determining the survival of pathologic cells in the circulation. In this paper we describe a sensitive technique, based on PS-dependent prothrombinase complex activity, for assessing the amount of PS on the external leaflet of intact viable cells. Our results indicate that tumorigenic, undifferentiated murine erythroleukemic cells express 7- to 8-fold more PS in their outer leaflet than do their differentiated, nontumorigenic counterparts. Increased expression of PS in the tumorigenic cells directly correlated with their ability to be recognized and bound by macrophages.

AB - Phosphatidylserine (PS) is asymmetrically distributed in mammalian cell membrane, being preferentially localized in the inner leaflet. Some studies have suggested that a disturbance in the normal asymmetric distribution of PS - e.g., PS exposure in the outer leaflet of the cell membrane, which can occur upon platelet activation as well as in certain pathologic red cells - serves as a potent procoagulant surface and as a single for triggering their recognition by macrophages. These studies suggest that the regulation of PS distribution in cell membranes may be critical in controlling coagulation and in determining the survival of pathologic cells in the circulation. In this paper we describe a sensitive technique, based on PS-dependent prothrombinase complex activity, for assessing the amount of PS on the external leaflet of intact viable cells. Our results indicate that tumorigenic, undifferentiated murine erythroleukemic cells express 7- to 8-fold more PS in their outer leaflet than do their differentiated, nontumorigenic counterparts. Increased expression of PS in the tumorigenic cells directly correlated with their ability to be recognized and bound by macrophages.

UR - http://www.scopus.com/inward/record.url?scp=0345112372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345112372&partnerID=8YFLogxK

M3 - Article

C2 - 2717615

AN - SCOPUS:0345112372

VL - 86

SP - 3184

EP - 3188

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 9

ER -